Resveratrol inhibits nitric oxide and TNF-α production by lipopolysaccharide-activated microglia
Resveratrol inhibits nitric oxide and TNF-α production by lipopolysaccharide-activated microglia
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DOI：
10.1016/j.intimp.2004.08.008
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发表时间：
2005-01-01
影响因子：
5.6
通讯作者：
Wu, CF
中科院分区：
文献类型：
--
作者：
Bi, XL;Yang, JY;Wu, CF
脑巨噬细胞作为小胶质细胞被激活后，释放促炎介质，在引发与神经退行性疾病相关的神经炎性反应中发挥重要作用。白藜芦醇是葡萄中的一种抗氧化成分，已有报道对巨噬细胞具有抗炎作用，因此我们研究了白藜芦醇对内毒素(LPS)激活的小胶质细胞产生肿瘤坏死因子-α(TNF-α)和一氧化氮(NO)的影响。培养的大鼠皮质小胶质细胞和小鼠小胶质细胞株N9暴露于脂多糖后，其释放的肿瘤坏死因子-α和一氧化氮增加，白藜芦醇可显著抑制其释放。进一步研究发现，白藜芦醇可抑制脂多糖诱导的N9小胶质细胞的IkappaBalpha降解、NOS表达和p38丝裂原活化蛋白激酶(MAPKs)的磷酸化。这些结果表明，白藜芦醇对小胶质细胞的促炎反应有很强的抑制作用，这表明该化合物在伴随小胶质细胞激活的神经退行性疾病中具有治疗潜力。(C)2004爱思唯尔B.V.保留所有权利。
Upon activation, brain macrophages, the microglia, release proinflammatory mediators that play important roles in eliciting neuroinflammatory responses associated with neurodegenerative diseases. As resveratrol, an antioxidant component of grape, has been reported to exert anti-inflammatory activities on macrophages, we investigated its effects on the production of TNF-alpha (TNF-alpha) and nitric oxide (NO) by lipopolysaccharide (LPS)-activated microglia. Exposure of cultured rat cortical microglia and a mouse microglial cell line N9 to LPS increased their release of TNF-alpha and NO, which was significantly inhibited by resveratrol. Further studies revealed that resveratrol suppressed LPS-induced degradation of IkappaBalpha, expression of NOS and phosphorylation of p38 mitogen-activated protein kinases (MAPKs) in N9 microglial cells. These results demonstrate a potent suppressive effect of resveratrol on proinflammatory responses of microglia, suggesting a therapeutic potential for this compound in neurodegenerative diseases accompanied by microglial activation. (C) 2004 Elsevier B.V. All rights reserved.