Inkjet monitoring technique with quartz crystal microbalance (QCM) sensor for highly reproducible antibody immobilization
Inkjet monitoring technique with quartz crystal microbalance (QCM) sensor for highly reproducible antibody immobilization
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DOI：
10.1016/j.sna.2014.08.010
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发表时间：
2014-11-01
影响因子：
4.6
通讯作者：
Ooie, T.
中科院分区：
文献类型：
--
作者：
Fuchiwaki, Y.;Yabe, Y.;Ooie, T.
我们证明，使用商用的石英晶体微天平(QCM)和压电喷墨喷头(喷射孔周围有一个平坦的表面)可以微沉积出校准数量的生物分子。QCM由不锈钢盖覆盖，不锈钢盖上有精密加工的孔，大大降低了由于气流和温度波动等外部来源造成的背景噪音，并允许在恒温下准确地喷射液滴。使用压电注射器将抗C反应蛋白抗体溶液(抗C反应蛋白；1.0 mg/ml)连续喷射到微通道靶上；使用30 MHz QCM逐点监测其微沉积。安装穿孔不锈钢盖后，QCM频率变化的离散度从7.87赫兹降至1.01赫兹。一抗准确沉积在靶标上，重复性好。用这一新技术校准一抗沉降量时，用酶联免疫吸附试验(ELISA法)测量的发光变异从9.67%降至1.16%。(C)爱思唯尔出版的2014年。
We demonstrate that a calibrated amount of a biomolecule can be microdeposited using a commercially available quartz crystal microbalance (QCM) and a piezoelectric inkjet head with a flattened surface surrounding the ejection hole. Covering the QCM with a stainless-steel cover perforated with a precisely-machined hole both significantly decreased background noise due to external sources such as air flow and temperature fluctuations and allowed accurate droplet ejection at a constant temperature. Anti-c reactive protein antibody solution (anti-CRP; 1.0 mg/ml) was continuously ejected onto a microchannel target using a piezoelectric injector; its microdeposition was monitored shot-by-shot using a 30 MHz QCM. The dispersion of the QCM frequency change per shot decreased from 7.87 to 1.01 Hz when a perforated stainless-steel cover was installed. The primary antibody was precisely deposited on the target with high reproducibility. The variation in luminescence measured by enzyme-linked immunosorbant assay (ELISA) decreased from 9.67% to 1.16% when calibrating the amount of primary antibody deposition with this developed technique. (C) 2014 Published by Elsevier B.V.