Immunocytochemical detection of HPV high-risk type L1 capsid proteins in LSIL and HSIL as compared with detection of HPV L1 DNA
Immunocytochemical detection of HPV high-risk type L1 capsid proteins in LSIL and HSIL as compared with detection of HPV L1 DNA
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DOI：
10.1159/000326491
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发表时间：
2003-03-01
期刊：
影响因子：
1.8
通讯作者：
Bastert, G
中科院分区：
文献类型：
--
作者：
Melsheimer, P;Kaul, S;Bastert, G
目的：探讨HPV L1衣壳蛋白在单纯疱疹病毒(HPV)感染的单纯疱疹病毒(HPV)感染的HSIL和LSIL中的分布情况。研究设计：前瞻性收集经细胞学和组织学证实的LIL(n=32)和HSIL(n=42)宫颈涂片，用标准化的L1通用引物聚合酶链式反应(My 09/11)检测HPV高危型(Hr)16、18、33、35、39、45、56和58 L1-DNA，用免疫细胞化学方法检测L1衣壳蛋白。HPVH1DNA阳性率分别为93%和59%，HPV16型分别为69%和37%。HSIL和LSIL中HPVHr33%和44%阳性，HPV16阳性29%和31%。HPV hrL1DNA阳性HSIL的L1衣壳蛋白表达显著降低，分别为59.6%和40.4%(P<0.01)。在HPV16DNA阳性和HPVhrDNA阳性的HSIL中，相应的L1衣壳蛋白的表达没有明显的下降。结论：HPV16型和HPVhrDNA感染的HSIL中病毒细胞相互作用受到干扰，L1衣壳抗原丢失。在这方面，T31和T16可能作为CIN预后标志物来预测CIN的预后。
OBJECTIVE: To investigate the prevalence of HPV L1 capsid proteins in HPV-infected HSIL and LSIL.STUDY DESIGN: Cervical smears from 74 women with cytologically and histologically confirmed LSIL (n=32) and HSIL (n=42) were collected prospectively to detect HPV high-risk (hr) types 16, 18, 33, 35, 39, 45, 56 and 58 L1-DNA by standardized L1-consensus primer PCR (MY 09/11) and L1 capsid proteins by immunoocytochemistry using monoclonal antibodies T31 (HPV16) and T16 (HPV hr) in a standardized protocol.RESULTS: In HSIL and LSIL, L1 DNA was found for HPV hr in 93% and 59% and for HPV16 in 69% and 37% of the specimens, respectively. HPV L1 capsid proteins were detected in HSIL and LSIL for HPV hr in 33% and 44% and for HPV16 in 29% and 31% of the specimens, respectively. Expression of L1 capsid proteins was significantly reduced, by 59.6% for HPV hr L1 DNA-positive HSIL (P < .01) and by 40.4% for HPV 16 L1 DNA-positive HSIL (P < .01). In HPV 16 DNA-positive and HPV hr DNA-positive LSIL, no significant reduction of corresponding L1 capsid protein expression could be demonstrated.CONCLUSION: These data suggest a disturbed viral cellular interaction in HPV 16 and HPV hr-infected HSIL, with loss of viral L1 capsid antigen. In this context there is a possible role of T31 and T16 as prognostic markers to predict the prognosis of CIN.