Determination of 5'-bromodeoxyuridine in DNA by buoyant density.
Determination of 5'-bromodeoxyuridine in DNA by buoyant density.
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通过浮力密度测定 DNA 中的 5-溴脱氧尿苷。
DOI：
10.1016/0003-2697(77)90247-0
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发表时间：
1977
影响因子：
2.9
通讯作者：
M. Bick
中科院分区：
文献类型：
--
作者：
D. Luk;M. Bick
氯化铯浮力密度分析显示，dT和dA的双链交替共聚物poly（dAT·dAT）和dA和BrdU的双链交替共聚物（dABU·dABU）之间的密度差为0.1730 g/cm 3。用5′-溴脱氧尿苷（BrdU）完全取代的哺乳动物DNA的密度比对照的未取代DNA的密度增加，增加值在共聚物分析预测值的1%以内。一个简单的关系，提出了准确测定的程度BrdU取代的DNA的异质性碱基组合物。
Cesium chloride buoyant density analysis shows the density difference between the double-stranded alternating copolymers of dT and dA, poly(dAT·dAT) and of dA and BrdU, (dABU·dABU), to be 0.1730 g/cm3. A mammalian DNA fully substituted with 5′-bromodeoxyuridine (BrdU) increases in density over control, unsubstituted, DNA by a value within 1% of that predicted from analysis of the copolymers. A simple relationship is presented for the accurate determination of the extent of BrdU substitution in a DNA of heterogeneous base composition.