Mouse cysteine-rich secretory protein 4 (CRISP4): A member of the crisp family exclusively expressed in the epididymis in an androgen-dependent manner
Mouse cysteine-rich secretory protein 4 (CRISP4): A member of the crisp family exclusively expressed in the epididymis in an androgen-dependent manner
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DOI：
10.1095/biolreprod.104.035758
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发表时间：
2005-05-01
影响因子：
3.6
通讯作者：
Poutanen, M
中科院分区：
文献类型：
--
作者：
Jalkanen, J;Huhtaniemi, P;Poutanen, M
在睾丸中产生的精子的最终成熟发生在它们通过附睾体的过程中。在这个过程中，分泌到附睾腔的蛋白质随着附睾液的pH和盐组成的变化而引起精子质膜的几个生化变化和重塑。Crisp家族是一组由三个成员组成的富含半胱氨酸的分泌蛋白，其中CRISP1是一种附睾蛋白，可以附着在附睾腔内的精子表面，并抑制配子膜的融合。在本文中，我们介绍了Crisp蛋白家族的一个新成员，CRISP4。这个新基因是通过对Uni-gene数据库中的附睾体表达序列标签文库进行电子分析而发现的。CRISP4的多肽序列有一个信号序列，表明它被分泌到附睾腔，因此可能与精子相互作用。与该家族的其他成员不同，Crisp4位于1号染色体上，位于一组编码富含半胱氨酸的蛋白质的基因中。Crisp4在小鼠体内以雄激素依赖的方式在附睾上皮细胞中特异表达，随着精子成熟的开始，该基因的表达开始于青春期。所鉴定的小鼠CRISP4多肽与人CRISP1有很高的同源性，且同源性高于人和小鼠的CRISP1，提示CRISP4代表人CRISP1的小鼠对精子膜的作用，可能与小鼠和人的CRISP1相似。
The final maturation of spermatozoa produced in the testis takes place during their passage through the epididymis. In this process, the proteins secreted into the epididymal lumen along with changes in the pH and salt composition of the epididymal fluid cause several biochemical changes and remodeling of the sperm plasma membrane. The Crisp family is a group of cysteinerich secretory proteins that previously consisted of three members, one of which-CRISP1-is an epididymal protein shown to attach to the sperm surface in the epididymal lumen and to inhibit gamete membrane fusion. In the present paper, we introduce a new member of the Crisp protein family, CRISP4. The new gene was discovered through in silico analysis of the epididymal expressed sequence tag library deposited in the Uni-Gene database. The peptide sequence of CRISP4 has a signal sequence suggesting that it is secreted into the epididymal lumen and might thus interact with sperm. Unlike the other members of the family, Crisp4 is located on chromosome 1 in a cluster of genes encoding for cysteine-rich proteins. Crisp4 is expressed in the mouse exclusively in epithelial cells of the epididymis in an androgen-dependent manner, and the expression of the gene starts at puberty along with the onset of sperm maturation. The identified murine CRISP4 peptide has high homology with human CRISP1, and the homology is higher than that between murine and human CRISP1, suggesting that CRISP4 represents the mouse counterpart of human CRISP1 and could have similar effects on sperm membrane as mouse and human CRISP1.