Thiol-yne radical reaction mediated site-specific protein labeling via genetic incorporation of an alkynyl-L-lysine analogue
Thiol-yne radical reaction mediated site-specific protein labeling via genetic incorporation of an alkynyl-L-lysine analogue
复制标题
硫醇-炔自由基反应通过炔基-L-赖氨酸类似物的遗传掺入介导位点特异性蛋白质标记。
DOI：
10.1039/c3ob27116a
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发表时间：
2013-01-01
影响因子：
3.2
通讯作者：
Guo, Qingxiang
中科院分区：
文献类型：
--
作者：
Li, Yiming;Pan, Man;Guo, Qingxiang
利用PylRS-tRNA对突变体高效合成了3个含炔吡咯赖氨酸衍生物，并将其编码为蛋白质。有了这些炔手柄，可以通过生物正交巯基炔连接反应实现蛋白质的位点特异性双标记。
Three alkyne-containing pyrrolysine derivatives were synthesized and genetically encoded into proteins by a mutant PylRS-tRNA pair with high efficiencies. With these alkyne handles, site-specific dual labeling of proteins can be achieved via a bioorthogonal thiol-yne ligation reaction.