THE FISSION YEAST DIS3+ GENE ENCODES A 110-KDA ESSENTIAL PROTEIN IMPLICATED IN MITOTIC CONTROL
THE FISSION YEAST DIS3+ GENE ENCODES A 110-KDA ESSENTIAL PROTEIN IMPLICATED IN MITOTIC CONTROL
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DOI：
10.1128/mcb.11.12.5839
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发表时间：
1991-12-01
影响因子：
5.3
通讯作者：
YANAGIDA, M
中科院分区：
文献类型：
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作者：
KINOSHITA, N;GOEBL, M;YANAGIDA, M
裂殖酵母突变体dis 3 -54在有丝分裂中有缺陷并且在染色体分离中失败。 其表型与dis 2 -11相似，dis 2 -11是一种在1型蛋白磷酸酶基因中发生突变的突变体。 我们通过转化法克隆了dis 3+基因。 核苷酸测序预测970个氨基酸的编码区，在第65位密码子处被164 bp的内含子中断。 预测的dis 3+蛋白与芽殖酵母SSD 1或SRK 1基因产物具有弱但显著的相似性，所述基因是蛋白磷酸酶SIT 4基因或环AMP依赖性蛋白激酶的BCY 1调节亚基的缺失的抑制剂。 抗dis 3抗体识别110-kDa的dis 3+基因产物，其是250- 350-kDa寡聚体的一部分，并且在细胞核中富集。 dis 3+蛋白的细胞定位与dis 2+蛋白相似，但这两种蛋白不形成复合物。 dis 3 -54突变体提取物中的1型蛋白磷酸酶活性显然不受影响。 dis 3+基因是生长所必需的;基因破坏细胞不发芽，细胞分裂失败。 增加dis 3+基因剂量逆转cdc 25 weel菌株的Ts+表型，增加1型蛋白磷酸酶基因剂量也是如此。 双突变体dis 3 dis 2即使在允许温度下也是致死的，这表明dis 2+和dis 3+基因可能在功能上重叠。 dis 3+基因产物在有丝分裂中的作用尚不清楚，但该基因产物可能直接或间接参与有丝分裂的调节。
The fission yeast mutant dis3-54 is defective in mitosis and fails in chromosome disjunction. Its phenotype is similar to that of dis2-11, a mutant with a mutation in the type 1 protein phosphatase gene. We cloned the dis3+ gene by transformation. Nucleotide sequencing predicts a coding region of 970 amino acids interrupted by a 164-bp intron at the 65th codon. The predicted dis3+ protein shares a weak but significant similarity with the budding yeast SSD1 or SRK1 gene product, the gene for which is a suppressor for the absence of a protein phosphatase SIT4 gene or the BCY1 regulatory subunit of cyclic AMP-dependent protein kinase. Anti-dis3 antibodies recognized the 110-kDa dis3+ gene product, which is part of a 250- to 350-kDa oligomer and is enriched in the nucleus. The cellular localization of the dis3+ protein is reminiscent of that of the dis2+ protein, but these two proteins do not form a complex. A type 1 protein phosphatase activity in the dis3-54 mutant extracts is apparently not affected. The dis3+ gene is essential for growth; gene disruptant cells do not germinate and fail in cell division. Increased dis3+ gene dosage reverses the Ts+ phenotype of a cdc25 weel strain, as does increased type 1 protein phosphatase gene dosage. Double mutant dis3 dis2 is lethal even at the permissive temperature, suggesting that the dis2+ and dis3+ genes may be functionally overlapped. The role of the dis3+ gene product in mitosis is unknown, but this gene product may be directly or indirectly involved in the regulation of mitosis.