Seroepidemiology of human bocavirus in Hokkaido Prefecture, Japan
Seroepidemiology of human bocavirus in Hokkaido Prefecture, Japan
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DOI：
10.1128/jcm.02140-06
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发表时间：
2007-10-01
影响因子：
9.4
通讯作者：
Ariga, Tadashi
中科院分区：
文献类型：
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作者：
Endo, Rika;Ishiguro, Nobuhisa;Ariga, Tadashi
2005年，瑞典研究人员发现了一种新的人类病毒，暂时命名为人类博卡病毒（HBoV）。建立了一种新的免疫荧光检测方法，用表达乙型肝炎病毒（HBoV）VP 1蛋白的重组杆状病毒感染粉纹夜蛾（Trichoplusia ni）昆虫细胞，检测血清中抗HBoV VP 1蛋白的IgG抗体水平。在0个月至41岁的日本人群中，针对HBoV VP 1蛋白的抗体的总体血清阳性率为71.1%（145/204）。6 ~ 8月龄组血清阳性率最低，随年龄增长逐渐升高。所有儿童在6岁时都接触过HBoV。4例下呼吸道感染患者在恢复期血清抗HBoVVP 1蛋白抗体滴度升高，鼻咽拭子和血清标本均检出HBoVDNA。这些结果表明，HBoV是一种普遍存在的病毒，在生命早期获得，HBoV可能在下呼吸道感染的过程中发挥作用。
A new human virus, provisionally named human bocavirus (HBoV), was discovered by Swedish researchers in 2005. A new immunofluorescence assay using Trichoplusia ni insect cells infected with a recombinant baculovirus expressing the VP1 protein of HBoV was developed, and the levels of immunoglobulin G antibody to the VP1 protein of HBoV in serum samples were measured. The overall seroprevalence rate of antibodies against the VP1 protein of HBoV in a Japanese population aged from 0 months to 41 years was 71.1% (145 of 204). The seropositive rate was lowest in the age group of 6 to 8 months and gradually increased with age. All of the children had been exposed to HBoV by the age of 6 years. A rise in titers of antibody against the VP1 protein of HBoV during the convalescent phase was observed for four patients with lower respiratory tract infections, and HBoV DNA was detected in nasopharyngeal swab and serum samples from all four patients. These results suggest that HBoV is a ubiquitous virus acquired early in life and that HBoV might play a role in the course of lower respiratory tract infections.