Procalcitonin and CGRP-I mRNA expression in various human tissues
Procalcitonin and CGRP-I mRNA expression in various human tissues
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DOI：
10.1097/00024382-200116020-00004
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发表时间：
2001-08-01
期刊：
影响因子：
3.1
通讯作者：
Reinhart, K
中科院分区：
文献类型：
--
作者：
Russwurm, S;Stonans, I;Reinhart, K
降钙素原（PCT）是一种高度敏感和特异性的全身性细菌感染和败血症标志物。与其诊断意义相反，血浆降钙素原的细胞来源仍有待澄清。两种形式的PCT mRNA来源于降钙素/降钙素基因相关肽基因（CALC-I基因）和降钙素基因相关肽- i (CGRP-I) mRNA。利用新设计的引物逆转录聚合酶链反应检测不同的PCT mRNA和CGRP-I mRNA，以鉴定可能参与PCT产生的组织。我们的研究表明，各种人体组织（总体分析的16个组织中的13个）表达PCT-I、PCT-II和/或cgrp - 1 mrna，在肝脏、睾丸、肺、前列腺、肾脏和小肠中检测到的水平最高。不同组织中PCT-I、PCT-II和CGRP-I mRNA的表达比例不同。因此，我们证明了CALC-I基因表达的组织特异性调控的复杂性，并假设多种组织是CALC-I编码肽的潜在来源。
Procalcitonin (PCT) is a highly sensitive and specific marker of systemic bacterial infection and sepsis. In contrast to Its diagnostic significance, the cellular sources of plasma procalcitonin remain to be clarified. Two forms of PCT mRNAs originate from calcitonin/calcitonin gene-related peptide gene (CALC-I gene) along with mRNA for calcitonin gene-related peptide-I (CGRP-I). Reverse transcription polymerase chain reaction with newly designed primers detecting different PCT mRNAs and CGRP-I mRNA was used to identify tissues that might contribute to PCT production. Our study indicates that a variety of human tissues (13 of the 16 analyzed overall) express PCT-I, PCT-II, and/or CGRP-l mRNAs, with the highest levels detected for liver, testis, lung, prostate, kidney, and small intestine. Various tissues differ in the proportions of PCT-I, PCT-II, and CGRP-I mRNA expression levels. Thus we demonstrate the complexity of tissue-specific regulation of CALC-I gene expression and suppose a variety of tissues as a potential source of CALC-I-encoded peptides.