Skeletogenic fate of zebrafish cranial and trunk neural crest.
Skeletogenic fate of zebrafish cranial and trunk neural crest.
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斑马鱼颅和躯干神经波峰的骨骼发明命运。
DOI：
10.1371/journal.pone.0047394
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Fisher S
中科院分区：
文献类型：
--
作者：
Kague E;Gallagher M;Burke S;Parsons M;Franz-Odendaal T;Fisher S
神经嵴（NC）是脊椎动物颅面骨骼的主要贡献者，通过胚胎学和遗传学方法在模型生物中详细描述，最显著的是鸡和小鼠。尽管这些相当遥远的物种之间有许多相似之处，但NC的贡献也有明显的差异，特别是对头骨的头盖骨。缺乏其他脊椎动物群体的信息排除了对这些差异的进化意义的理解。斑马鱼颅面发育的研究对了解硬骨鱼类软骨和骨的形成有很大的贡献，但目前关于NC对斑马鱼骨骼的贡献的信息很少。在这里，我们采用了两个转基因系统的基础上Cre重组酶的遗传标记NC在斑马鱼。我们证明NC贡献的细胞在颅神经节和周围神经系统已知是NC衍生的，以及心肌细胞的一个子集。不可磨灭的标记也使我们能够确定NC的贡献，后期形成的骨骼，包括颅骨。我们确认怀疑NC起源的软骨和骨的内脏颅，包括软骨，如hyosymplectic和它的替代骨（hymandibula和辛）和膜骨，如鳃盖骨。匙突发育在NC和中胚层的边界处，并且作为胸带的祖先组成部分，被预测为由NC和中胚层组织组成的混合骨。然而，我们没有发现任何证据表明，NC贡献的匙状突。同样地，在颅骨的穹窿中，顶骨和额骨的尾侧部分没有显示出NC贡献的证据。我们还确定了尾鳍lepidotrichia NC起源的假设是，这些都是来自躯干NC，表明这些细胞有能力在正常的脊椎动物发育过程中形成骨。
The neural crest (NC) is a major contributor to the vertebrate craniofacial skeleton, detailed in model organisms through embryological and genetic approaches, most notably in chick and mouse. Despite many similarities between these rather distant species, there are also distinct differences in the contribution of the NC, particularly to the calvariae of the skull. Lack of information about other vertebrate groups precludes an understanding of the evolutionary significance of these differences. Study of zebrafish craniofacial development has contributed substantially to understanding of cartilage and bone formation in teleosts, but there is currently little information on NC contribution to the zebrafish skeleton. Here, we employ a two–transgene system based on Cre recombinase to genetically label NC in the zebrafish. We demonstrate NC contribution to cells in the cranial ganglia and peripheral nervous system known to be NC–derived, as well as to a subset of myocardial cells. The indelible labeling also enables us to determine NC contribution to late–forming bones, including the calvariae. We confirm suspected NC origin of cartilage and bones of the viscerocranium, including cartilages such as the hyosymplectic and its replacement bones (hymandibula and symplectic) and membranous bones such as the opercle. The cleithrum develops at the border of NC and mesoderm, and as an ancestral component of the pectoral girdle was predicted to be a hybrid bone composed of both NC and mesoderm tissues. However, we find no evidence of a NC contribution to the cleithrum. Similarly, in the vault of the skull, the parietal bones and the caudal portion of the frontal bones show no evidence of NC contribution. We also determine a NC origin for caudal fin lepidotrichia; the presumption is that these are derived from trunk NC, demonstrating that these cells have the ability to form bone during normal vertebrate development.