Involvement of calpain in melanogenesis of mouse B16 melanoma cells
Involvement of calpain in melanogenesis of mouse B16 melanoma cells
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DOI：
10.1007/s11010-005-1081-0
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发表时间：
2005-07-01
影响因子：
4.3
通讯作者：
Nozawa, Y
中科院分区：
文献类型：
--
作者：
Ohguchi, K;Akao, Y;Nozawa, Y
在目前的研究中，钙蛋白酶，半胱氨酸蛋白酶在黑素生成的调节中的参与使用小鼠B16黑色素瘤细胞进行了检查。为了响应α-黑素细胞刺激激素（α-MSH），B16黑色素瘤细胞经历了以黑色素生物合成增加为特征的分化。α-MSH处理后2 h内总calapain活性下降，6-12 h恢复到初始水平。为了进一步研究钙蛋白酶参与黑素生成的调节，检查了钙蛋白酶抑制剂对α-MSH诱导的黑素生成的作用。通过N-乙酰基-Leu-Leu-norleucinal（ALLN）或钙蛋白酶抑制剂（CS）肽抑制钙蛋白酶阻断α-MSH诱导的黑素生成。黑色素生物合成的抑制程度与酪氨酸酶活性的降低密切相关，酪氨酸酶是黑色素生成中的关键调节酶。ALLN处理B16细胞后酪氨酸酶蛋白和mRNA水平均明显下降。这些结果表明钙蛋白酶可能通过调节酪氨酸酶的表达参与小鼠B16黑色素瘤细胞的黑素生成信号转导。
In the current study, the involvement of calpain, a cysteine proteinase in the regulation of melanogenesis was examined using mouse B16 melanoma cells. In response to alpha-melanocyte-stimulating hormone (alpha-MSH), B16 melanoma cells underwent differentiation characterized by increased melanin biosynthesis. The total calapain activity was decreased within 2 h following alpha-MSH-treatment, and restored to the initial level in 6-12 h. To further investigate the involvement of calpain in the regulation of melanogenesis, the effect of calpain inhibitors on alpha-MSH-induced melanogenesis was examined. Inhibition of calpain by either N-acetyl-Leu-Leu-norleucinal (ALLN) or calpastatin (CS) peptide blocked alpha-MSH-induced melanogenesis. The magnitude of inhibition of melanin biosynthesis was well correlated with a decrease in the activity of tyrosinase, a key regulatory enzyme in melanogenesis. Treatment of B16 cells with ALLN caused marked decrease in both tyrosinase protein and mRNA levels. These results indicate that calpain would be involved in the melanogenic signaling by modulating the expression of tyrosinase in mouse B16melanoma cells.