A culture system enabling in situ determination of net and gross photosynthesis, O2 evolution, N assimilation, and C2H2 reduction in cyanobacteria
A culture system enabling in situ determination of net and gross photosynthesis, O2 evolution, N assimilation, and C2H2 reduction in cyanobacteria
复制标题
一种能够原位测定蓝藻净光合作用和总光合作用、O2 演化、N 同化和 C2H2 还原的培养系统
作者：
D. Turpin;D. Layzell
描述了一种恒化培养系统，其中测量与碳和氮同化相关的速率可以在固氮蓝藻的原位稳态培养中进行。净C和N同化是由稳态细胞碳和氮含量以及培养物的稳态生长速率来确定的。在无扰动培养皿中同时测量光下O2的演化和短期14C的同化。在20分钟的采样周期内，14C同化与时间呈线性关系，从而提供了总光合作用的度量。使用开放气体分析系统在培养皿内测量C2H2的还原。C2H2的稳定还原速率在10分钟内获得，并保持恒定长达1小时。初步结果表明，在相同的生长速率（0.018 ~ 0.019 h−1）下，与在NH3上生长的细胞相比，在N2上生长的细胞含有异囊，体积更小，叶绿素含量更高。相应的毛…
A chemostat culture system is described in which measurements of rates associated with carbon and nitrogen assimilation may be made in in situ steady-state cultures of N2-fixing cyanobacteria. Net C and N assimilations were determined from the steady-state cellular carbon and nitrogen contents and the steady-state growth rate of the cultures. O2 evolution in the light and short-term 14C assimilation were measured simultaneously in unperturbed culture cuvettes. 14C assimilation was linear with time through the origin over the 20-min sampling period, thereby providing a measure of gross photosynthesis. C2H2 reduction was measured within the culture cuvette using an open gas analysis system. Steady-state rates of C2H2 reduction were obtained within 10 min and remained constant for up to 1 h. Preliminary results showed that at similar growth rates (0.018–0.019 h−1) cultures grown on and N2 contained heterocysts, were smaller, and had higher chlorophyll contents than cells grown on NH3. Corresponding gross pho...