The microbial synergy of Peptostreptococcus micros and Prevotella intermedia in a murine abscess model
The microbial synergy of Peptostreptococcus micros and Prevotella intermedia in a murine abscess model
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DOI：
10.1111/j.0902-0055.2004.00138.x
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发表时间：
2004-06-01
影响因子：
--
通讯作者：
Karasawa, T
中科院分区：
文献类型：
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作者：
Araki, H;Kuriyama, T;Karasawa, T
本研究的特点是微生物相互作用的微小消化链霉菌和中间普雷沃菌，主要病原体的牙槽感染，使用小鼠模型。在小鼠背部皮下注射微小毕赤酵母细胞和中间毕赤酵母活细胞后，脓肿面积明显大于单次注射（P <0.02）。将微小毕赤酵母细胞悬液与中间毕赤酵母培养滤液一起注入小鼠体内，脓肿体积也明显增大（P <0.05）。热处理的中间青霉培养滤液也能增强微小青霉的毒力。微小变形杆菌培养滤液对中间变形杆菌的毒力无影响。有趣的是，即使将中间青霉的培养滤液注射到小鼠的不同部位，微小青霉的毒力似乎也会增强。这些结果表明，中间青霉的一种或多种热稳定产物通过改变宿主条件间接地增强了微小青霉的毒力，而微小青霉的活细胞可以直接增强中间青霉的毒力。
This study characterized the microbial interaction of Peptostreptococcus micros and Prevotella intermedia, the major pathogens of dentoalveolar infection, using a murine model. Subcutaneous injection of P. micros cells in the dorsum of the mouse together with living cells of P. intermedia resulted in a significantly larger abscess when compared with single injection of the organisms (P < 0.02). The abscess size was also significantly increased (P < 0.05) when the plate-cultured cell suspension of P. micros was injected into mouse with the culture filtrate of P. intermedia. The heat-treated culture filtrate of P. intermedia also enhanced the virulence of P. micros. P. micros culture filtrate did not affect the virulence of P. intermedia. Interestingly, the virulence of P. micros appeared to be enhanced even when the culture filtrate of P. intermedia was injected at separate sites in the mouse. These results suggest that a heat-stable product or products of P. intermedia increase the virulence of P. micros indirectly by altering the host condition, whereas living cells of P. micros can directly enhance virulence of P. intermedia.