IMMUNOGENETICS OF HUMAN MINOR HISTOCOMPATIBILITY ANTIGENS - THEIR POLYMORPHISM AND IMMUNODOMINANCE
IMMUNOGENETICS OF HUMAN MINOR HISTOCOMPATIBILITY ANTIGENS - THEIR POLYMORPHISM AND IMMUNODOMINANCE
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DOI：
10.1007/bf00185109
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发表时间：
1992-02-01
期刊：
影响因子：
3.2
通讯作者：
GOULMY, E
中科院分区：
文献类型：
--
作者：
VANELS, CACM;DAMARO, J;GOULMY, E
次要组织相容性 (mH) 抗原是多态性内源合成产物，可在主要组织相容性复合物分子的背景下被同种异体反应性 T 细胞识别。 在组织供体和受体的 HLA 匹配的移植情况下，mH 抗原可能会引发强烈的细胞免疫反应。 为了深入了解 mH 抗原的多态性，我们研究了它们在健康人群中的频率。 由不同的细胞毒性 T 细胞 (CTL) 克隆识别的五种 HLA I 类限制性 mH 抗原用于由一组 (N = 100) HLA 型靶细胞组成的群体遗传分析。 三种 mH 抗原显示出 69% 或更高的表型频率，这与其他两种 mH 抗原的频率分别为 16% 和 7% 形成鲜明对比。 为了深入了解 T 细胞对 mH 抗原反应的“功能性”多态性，我们分析了个体内 CTL 克隆的特异性。 接受调查的五分之三的人对一种 HLA-A2 限制性 mH 抗原有 CTL 反应。 这些结果表明健康人群中某些 mH 抗原的等位基因多态性有限，并提示免疫显性人类 mH 抗原的存在。
Minor Histocompatibility (mH) antigens are polymorphic endogenously synthesized products that can be recognized by alloreactive T cells in the context of major histocompatibility complex molecules. In transplant situations where tissue donor and recipient are matched for HLA, mH antigens may trigger strong cellular immune responses. To gain insight into the polymorphism of mH antigens we studied their frequencies in the healthy population. Five HLA class I restricted mH antigens recognized by distinct cytotoxic T-cell (CTL) clones were used in the population genetic analysis consisting of a panel (N = 100) of HLA typed target cells. Three mH antigens showed phenotype frequencies of 69% or higher, this contrasted the frequencies of two other mH antigens with 16 and 7% respectively. To gain insight into the "functional" polymorphism of the T-cell response to mH antigens, we analyzed the specificity of CTL clones within individuals. Three out of five individuals investigated shared a CTL response to one single HLA-A2 restricted mH antigen. These results indicate limited allelic polymorphism for some mH antigens in the healthy population and are suggestive of the existence of immunodominant human mH antigens.