Gene Expression Profiles in Murine Influenza Pneumonia
Gene Expression Profiles in Murine Influenza Pneumonia
复制标题
鼠流感肺炎的基因表达谱
DOI：
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发表时间：
2008
影响因子：
5.3
通讯作者：
T. van der Poll
中科院分区：
文献类型：
--
作者：
M. Dessing;K. F. van der Sluijs;C. Spek;T. van der Poll
背景：许多体外研究都集中在流感感染的白细胞、肺组织或细胞系中的基因表达。然而，对体内基因在这些隔室中的表达的了解是有限的。方法：为了深入了解流感感染过程中的基因表达谱，我们利用一种新开发的小鼠特异性多重连接依赖的探针扩增方法来检测多个基因的表达。结果：甲型流感鼻腔感染前后，小鼠肺组织、支气管肺泡灌洗液中的白细胞和气管上皮细胞中与炎症、Toll样受体信号转导、凝血、纤溶、细胞黏附、组织修复和稳态相关的基因均有表达，大多数基因在感染过程中有差异表达，在小鼠康复后恢复到基础水平。然而，尽管老鼠已经完全清除了病毒，但几个基因的表达仍然发生了变化。结论：这些数据为流感期间呼吸道基因表达谱提供了第一个信息。
Background: Many in vitro studies have focused on gene expression in influenza-infected leukocytes, lung tissue or cell lines. However, knowledge of in vivo gene expression in these compartments is limited. Methods: To obtain insight into gene expression profiles during influenza infection, we determined the expression of multiple genes by using a newly developed mouse-specific multiplex ligation-dependent probe amplification assay. Results: The genes involved in inflammation, Toll-like receptor signaling, coagulation, fibrinolysis, cell adhesion, tissue repair and homeostasis were measured in lung tissue, leukocytes in bronchoalveolar lavage fluid and tracheal epithelial cells in mice, before and after intranasal infection with influenza A. Most of the genes investigated were differentially expressed during the course of infection and returned to baseline levels when mice had recovered from the infection. However, expression of several genes remained altered even though mice had completely cleared the virus. Conclusion: These data provide the first information on compartmentalized gene expression profiles in the respiratory tract during influenza.
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影响因子：
3.8
作者：
H. Tong;J. P. Long;Daneng Li;T. Demaria
通讯作者：
H. Tong;J. P. Long;Daneng Li;T. Demaria
DOI：
10.1073/pnas.0400937101
发表时间：
2004-04-13
影响因子：
11.1
作者：
Lund, JM;Alexopoulou, L;Flavell, RA
通讯作者：
Flavell, RA
影响因子：
6
作者：
Dawson, TC;Beck, MA;Maeda, N
通讯作者：
Maeda, N
囊性纤维化和正常人气道上皮细胞对甲型流感病毒感染的反应。
DOI：
10.1089/jir.2006.26.609
发表时间：
2006
期刊：
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research
影响因子：
--
作者：
Xu,Weiling;Zheng,Shuo;Goggans,TannishiaM;Kiser,Patti;Quinones-Mateu,MiguelE;Janocha,AllisonJ;Comhair,SuzyAA;Slee,Roger;Williams,BryanRG;Erzurum,SerpilC
通讯作者：
Erzurum,SerpilC