DNA hybridization detection with blue luminescent quantum dots and dye-labeled single-stranded DNA
DNA hybridization detection with blue luminescent quantum dots and dye-labeled single-stranded DNA
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DOI：
10.1021/ja0685452
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发表时间：
2007-03-21
影响因子：
15
通讯作者：
Travas-Sejdic, Jadranka
中科院分区：
文献类型：
--
作者：
Peng, Hui;Zhang, Lijuan;Travas-Sejdic, Jadranka
基于蓝色发光 CdTe QD 和染料标记的 ssDNA 之间的荧光共振能量转移 (FRET)，开发了一个简单的 DNA 传感平台。阳离子聚合物充当“桥梁”，将供体与受体连接在一起，实现高 FRET 效率。由于单链和双链 DNA 与聚合物的相互作用能力不同，因此可以通过不同的 FRET 效率来识别杂交事件。该平台提供了一种均质 DNA 测定，利用荧光检测方法，但 DNA 修饰最少。
A simple DNA sensing platform was developed based on the fluorescence resonance energy transfer (FRET) between blue-luminescent CdTe QDs and dye-labeled ssDNA. A cationic polymer acts as a "bridge" to bring the donor to acceptor together and achieves the high FRET efficiency. The hybridization event was recognized by the different FRET efficiencies due to different interaction abilities of single-stranded and double-stranded DNA with the polymer. This platform provides a homogeneous DNA assay that takes advantage of the fluorescence detection method, but with minimal DNA modification.