Insulin and insulinlike growth factor receptors and responses in cultured human muscle cells.
Insulin and insulinlike growth factor receptors and responses in cultured human muscle cells.
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培养的人体肌肉细胞中的胰岛素和胰岛素样生长因子受体和反应。
DOI：
10.1152/ajpendo.1986.251.5.e611
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Roth,RA
中科院分区：
文献类型：
--
作者：
Shimizu,M;Webster,C;Morgan,DO;Blau,HM;Roth,RA
在培养的人类成肌细胞和肌管上发现了胰岛素样生长因子 I 和 II（IGF-I 和 IGF-II）的特异性受体。相比之下，肌管而非成肌细胞特异性结合胰岛素，并受到纳摩尔浓度胰岛素的刺激而吸收脱氧葡萄糖。此外，在成肌细胞中，生理浓度的IGF-I和-II以及在较小程度上的胰岛素刺激不可代谢氨基酸类似物甲氨基异丁酸（MAIB）的摄取两到三倍。在肌管中，MAIB 的摄取优先受到 IGF-I 的刺激。利用优先识别胰岛素受体或IGF-I受体的单克隆抗体来检查哪些受体介导这些激素的生物效应。胰岛素对成肌细胞和肌管的作用似乎部分由胰岛素受体介导，部分由 IGF-I 受体介导。在肌管中，IGF-I 和-II 的作用似乎都是通过 IGF-I 受体介导的。在成肌细胞中，两种 IGF 的作用似乎部分由 IGF-I 受体介导，部分由 IGF-II 受体或另一种类型的 IGF-I 受体介导。目前的结果表明，培养的人体肌肉细胞提供了一个有用的模型系统，可用于研究胰岛素和 IGF 的生物学作用。
Specific receptors for insulinlike growth factors I and II (IGF-I and IGF-II) were found on cultured human myoblasts and myotubes. In contrast, myotubes but not myoblasts specifically bound insulin and were stimulated by nanomolar concentrations of insulin to take up deoxyglucose. In addition, in myoblasts, physiological concentrations of IGF-I and -II and, to a lesser extent, insulin stimulated two- to threefold the uptake of the nonmetabolizable amino acid analogue methylaminoisobutyric acid (MAIB). In myotubes, uptake of MAIB was stimulated preferentially by IGF-I. Monoclonal antibodies that preferentially recognize either the insulin receptor or the IGF-I receptor were utilized to examine which receptors mediated the biological effects of these hormones. The effects of insulin on both myoblasts and myotubes appeared to be mediated in part by the insulin receptor and in part by the IGF-I receptor. In myotubes, the effects of IGF-I and -II both appeared to be mediated through the IGF-I receptor. In myoblasts, the effects of the two IGFs appeared to be in part mediated by the IGF-I receptor and in part mediated by either the IGF-II receptor or another type of IGF-I receptor. The present results suggest that cultured human muscle cells provide a useful model system in which to study the biological actions of insulin and the IGFs.