The genetic and molecular dissection of abscisic acid biosynthesis and signal transduction in Arabidopsis
The genetic and molecular dissection of abscisic acid biosynthesis and signal transduction in Arabidopsis
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DOI：
10.1016/s0981-9428(98)80093-4
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发表时间：
1998-01-01
影响因子：
6.5
通讯作者：
Zeevaart, JAD
中科院分区：
文献类型：
--
作者：
Koornneef, M;Léon-Kloosterziel, KM;Zeevaart, JAD
研究了植物激素脱落酸（ABA）在拟南芥中的作用，利用受该激素生物合成或作用方式影响的突变体。突变体主要是通过改变萌发特性和幼苗生长来分离的。鉴定了aba1、aba2和aba3突变体的生化损伤，并通过同源性克隆了aba1编码的玉米黄质环氧化酶基因，该基因与在同一生物合成步骤中被阻断的烟叶基因同源。ABA不敏感突变体要么具有影响多个ABA过程（abi1和abi2）的表型，因此被认为编码ABA信号转导的早期步骤，要么影响特定步骤（例如abi3， abi4, abi5）。ABI1和ABI2基因编码蛋白磷酸酶2C酶和ABI3是一种具有种子特异性表达的转录因子。ABA超敏er1突变体在法尼基转移酶中受损。不同的突变体被用来分析ABA在种子发育和萌发、胁迫耐受性和气孔关闭中的作用。(C)爱思唯尔，巴黎。
The role of the plant hormone abscisic acid (ABA) has been studied in Arabidopsis by using mutants affected in either the biosynthesis or the mode of action of this hormone. Mutants have been isolated mainly by altered germination characteristics and seedling growth. The biochemical lesions of the aba1, aba2 and aba3 mutants have been identified and the zeaxanthin epoxidase gene encoded by ABA1 has been cloned by homology with a Nicotiana plumbaginifolia gene blocked at the same biosynthetic step. ABA-insensitive mutants have either a phenotype affecting several ABA processes (abi1 and abi2) and therefore were suggested to encode early steps in ABA signal transduction, or they affect specific steps (e.g. abi3, abi4, abi5). The ABI1 and ABI2 genes encode protein phosphatase 2C enzymes and ABI3 a transcription factor with seed-specific expression. The ABA hypersensitive era1 mutant is impaired in a farnesyl transferase. The various mutants have been used to analyse the role of ABA in seed development and seed germination, stress tolerance, and stomatal closure. (C) Elsevier, Paris.