THE YEAST-CELL CYCLE GENE CDC34 ENCODES A UBIQUITIN-CONJUGATING ENZYME
THE YEAST-CELL CYCLE GENE CDC34 ENCODES A UBIQUITIN-CONJUGATING ENZYME
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DOI：
10.1126/science.2842867
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发表时间：
1988-09-09
期刊：
影响因子：
56.9
通讯作者：
BYERS, B
中科院分区：
文献类型：
--
作者：
GOEBL, MG;YOCHEM, J;BYERS, B
酵母CDC34基因突变体在细胞周期从G1期过渡到S期时存在缺陷。该基因被克隆并编码了一个295个残基的蛋白，该蛋白与酵母RAD6基因的产物具有很大的序列相似性。RAD6基因是多种细胞功能所必需的，包括DNA修复，最近被证明编码一种泛素结合酶。当在大肠杆菌中产生时，CDC34基因产物在体外催化了泛素与组蛋白H2A和H2B的共价结合，表明CDC34蛋白是泛素偶联酶家族的另一个独特成员。因此，CDC34的细胞周期功能可能是由其产物的泛素偶联活性介导的。
Mutants in the gene CDC34 o the yeast Saccharomyces cerevisiae are defective in the transition from G1 to the S phase of the cell cycle. This gene was cloned and shown to encode a 295-residue protein that has substantial sequence similarity to the product of the yeast RAD6 gene. The RAD6 gene is required for a variety of cellular functions including DNA repair and was recently shown to encode a ubiquitin-conjugating enzyme. When produced in Escherichia coli, the CDC34 gene product catalyzed the covalent attachement of ubiquitin to histones H2A and H2B in vitro, demonstrating that the CDC34 protein is another distinct member of the family of ubiquitinconjugating enzymes. The cell cycle function of CDC34 is thus likely to be mediated by the ubiquitin-conjugating activity of its product.