Wide-field subdiffraction imaging by accumulated binding of diffusing probes
Wide-field subdiffraction imaging by accumulated binding of diffusing probes
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DOI：
10.1073/pnas.0609643104
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发表时间：
2006-12-12
影响因子：
11.1
通讯作者：
Hochstrasser, Robin M.
中科院分区：
文献类型：
--
作者：
Sharonov, Alexey;Hochstrasser, Robin M.
介绍了一种通过碰撞通量累积点的亚衍射成像方法。它基于通过在溶液中扩散的荧光探针来瞄准物体的表面。由于物体上的探针通量在长时间内基本恒定，因此可以检查几乎无限数量的单个探针分子。通过用其质心处的点替换其点扩散函数，可以高精度地定位击中物体并固定不动的每个探针。显示了脂质双层的图像、这些双层的轮廓和大的单层囊泡。很容易实现大约 25 nm 的空间分辨率。该方法能够实现快速纳米级成像和低于瑞利标准的空间分辨率，并且无需使用荧光探针进行标记。
A method is introduced for subdiffraction imaging that accumulates points by collisional flux. It is based on targeting the surface of objects by fluorescent probes diffusing in the solution. Because the flux of probes at the object is essentially constant over long time periods, the examination of an almost unlimited number of individual probe molecules becomes possible. Each probe that hits the object and that becomes immobilized is located with high precision by replacing its point-spread function by a point at its centroid. Images of lipid bilayers, contours of these bilayers, and large unilamellar vesicles are shown. A spatial resolution of approximate to 25 nm is readily achieved. The ability of the method to effect rapid nanoscale imaging and spatial resolution below Rayleigh criterion and without the necessity for labeling with fluorescent probes is proven.