Correlation between ageing and collagen gel contractility of human fibroblasts.
Correlation between ageing and collagen gel contractility of human fibroblasts.
复制标题
衰老与人成纤维细胞胶原凝胶收缩力之间的相关性。
DOI：
10.2340/0001555570241244
复制
发表时间：
1990
影响因子：
3.6
通讯作者：
T. Hamada
中科院分区：
文献类型：
--
作者：
T. Kono;T. Tanii;M. Furukawa;N. Mizuno;J. Kitajima;M. Ishii;T. Hamada
为了研究衰老对伤口愈合的影响，我们在I型胶原凝胶中培养了来自人真皮的成纤维细胞，并评估了凝胶收缩性与衰老的关系。细胞分别取自儿童（0-15岁，A组）、成年早期（16-40岁，B组）、中年（41-60岁，C组）和老年（61岁及以上，D组）。凝胶制备后第2天通过测量直径来测定凝胶的收缩力。在第10代内，凝胶收缩在A组最为明显，但在其他组之间没有差异。30-40代凝胶收缩与B、C、D组第10代凝胶收缩无明显差异，但A组凝胶收缩明显减小，与其他组相似。这些结果表明，儿童时期的成纤维细胞比成年时期的成纤维细胞更具收缩性，更容易受到传代（体外老化）的影响。
To investigate the influence of ageing on wound healing, we cultured fibroblasts derived from human dermis in type I collagen gel, and evaluated the relationship between gel contractility and ageing. Cells were obtained from children (0-15 years old, Group A), early adulthood (16-40 years old, Group B), mid-adulthood (41-60 years old, Group C), and the elderly (61 or older, Group D). Gel contractility was determined by measuring the diameter on the second day after gel preparation. Within the tenth passage, gel contraction was the most marked in Group A, but did not differ among the other groups. Gel contraction at passages 30-40 did not differ from those within the tenth passage in Groups B, C and D, but it decreased markedly in Group A to a value similar to that in the other groups. These results show that fibroblasts in childhood are more contractile than those in adulthood and are more readily affected by passages (in vitro ageing).