ECTOPIC EXPRESSION OF THE SEROTONIN 1C RECEPTOR AND THE TRIGGERING OF MALIGNANT TRANSFORMATION
ECTOPIC EXPRESSION OF THE SEROTONIN 1C RECEPTOR AND THE TRIGGERING OF MALIGNANT TRANSFORMATION
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DOI：
10.1126/science.2727693
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发表时间：
1989-06-02
期刊：
影响因子：
56.9
通讯作者：
AXEL, R
中科院分区：
文献类型：
--
作者：
JULIUS, D;LIVELLI, TJ;AXEL, R
神经递质受体通常仅限于神经元细胞，但这些受体激活的信号通路广泛分布于神经细胞和非神经细胞中。研究人员检查了在成纤维细胞的非自然环境中激活大脑特异性神经递质受体（血清素 5HTlc 受体）的功能后果。将功能性5HT1c受体引入NIH 3T3细胞中，以高频率导致转化灶的产生。此外，转化灶的产生和维持需要血清素受体的持续激活。此外，将来自转化病灶的细胞注射到裸鼠体内会导致肿瘤的产生。因此，当在 NIH 3T3 成纤维细胞中表达时，血清素 5HTlc 受体充当原癌基因。
Neurotransmitter receptors are usually restricted to neuronal cells, but the signaling pathways activated by these receptors are widely distributed in both neural and non-neural cells. The functional consequences of activating a brain-specific neurotransmitter receptor, the serotonin 5HTlc receptor, in the unnatural environment of a fibroblast were examined. Introduction of functional 5HTlc receptors into NIH 3T3 cells results, at high frequency, in the generation of transformed foci. Moreover, the generation and maintenance of transformed foci requires continued activation of the serotonin receptor. In addition, the injection of cells derived from transformed foci into nude mice results in the generation of tumors. The serotonin 5HTlc receptor therefore functions as a protooncogene when expressed in NIH 3T3 fibroblasts.