Optimization of Schwann cell adhesion in response to shear stress in an in vitro model for peripheral nerve tissue engineering
Optimization of Schwann cell adhesion in response to shear stress in an in vitro model for peripheral nerve tissue engineering
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DOI：
10.1089/107632703764664701
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发表时间：
2003-04-01
影响因子：
--
通讯作者：
Gupta, RJ
中科院分区：
文献类型：
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作者：
Chafik, D;Bear, D;Gupta, RJ
神经引导通道（NGC）的设计正在不断发展，以产生一个有利于神经再生的环境。我们建立了一个体外模型，以评估这种改变的宿主环境的三个重要组成部分之间的相互作用：（1）雪旺细胞，（2）底物，和（3）以层流剪切应力形式的持续机械刺激。将融合前的施万细胞接种在涂有层粘连蛋白、聚D-赖氨酸、IV型胶原蛋白或纤连蛋白的载玻片上。将这些载玻片置于定制设计的平行板流动室中，并以15 mL/min的速率给予层流2 h。雪旺细胞粘附试验表明，层粘连蛋白（平均值，86.1%; SEM，4.47%）和纤连蛋白（平均值，81.7%; SEM，3.24%）在统计学上上级IV型胶原（平均值，57.7%; SEM，3.96%）和聚-D-赖氨酸（平均值，58.0%; SEM，4.97%）（p< 0.001）。纤连蛋白（平均12.20%; SEM，0.374%）比层粘连蛋白（平均8.14%; SEM，0.682%）诱导的雪旺细胞增殖更显著（p< 0.001）。因此，我们建议将纤连蛋白作为NGC的重要成分，并进行进一步的体内研究。由于机械应力是宿主环境的一个组成部分，我们的研究是第一个将此因素纳入外周神经组织工程体外模型。
The design of nerve guidance channels ( NGCs) is evolving to produce a favorable environment for neural regeneration. We created an in vitro model to evaluate the interactions between three centrally important components of this altered host environment: ( 1) Schwann cells, ( 2) substrate, and ( 3) sustained mechanical stimulus in the form of shear stress with laminar fluid flow. Preconfluent Schwann cells were plated on slides coated either with laminin, poly-D-lysine, type IV collagen, or fibronectin. These slides were placed into custom-designed, parallel-plate, flow chambers and were administered laminar fluid flow at a rate of 15 mL/min for 2 h. Schwann cell adhesion assays demonstrated that laminin (mean, 86.1%; SEM, 4.47%) and fibronectin (mean, 81.7%; SEM, 3.24%) were statistically superior to collagen type IV (mean, 57.7%; SEM, 3.96%) and poly-D-lysine ( mean, 58.0%; SEM, 4.97%) (p< 0.001). Fibronectin ( mean, 12.20%; SEM, 0.374%) induced statistically greater Schwann cell proliferation than did laminin ( mean, 8.14%; SEM, 0.682%) (p< 0.001). Therefore, we recommend that fibronectin should be used as an important component of NGCs with further in vivo studies. As mechanical stress is an integral part of the host environment, our study is the first to incorporate this factor into an in vitro model for peripheral nerve tissue engineering.