SEM imaging of the stimulatory response of RAW264.7 cells against Porphyromonas gingivalis using a simple technique employing new conductive materials.
SEM imaging of the stimulatory response of RAW264.7 cells against Porphyromonas gingivalis using a simple technique employing new conductive materials.
复制标题
使用新型导电材料的简单技术对 RAW264.7 细胞针对牙龈卟啉单胞菌的刺激反应进行 SEM 成像。
DOI：
10.1002/jbm.b.33940
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Yamamoto H.
中科院分区：
文献类型：
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作者：
Takahashi C;Umemura Y;Naka A;Yamamoto H.
在医学生物学中，不仅要了解生物的形态学，而且要了解生物材料和制剂之间的相互作用。为了研究这些，通常使用电子显微镜（EM）。然而，EM的样品制备技术需要高水平的技能和相当长的时间。在这里，我们使用使用导电液体BEL-1的简单技术进行EM，并将结果与使用离子液体1-丁基-3-甲基咪唑四氟硼酸盐（[BMIM][BF 4]）的另一种简单技术进行比较。BEL-1用于样品预处理，通过与P.使用场发射扫描电镜（FE-SEM）观察牙龈炎。在本研究中，炎症诱导系统的P.成功建立牙龈炎。FE-SEM结果显示RAW 264.7细胞系和P. Gingivalisand证实了P引起的RAW 264.7细胞系的形态学变化。牙龈炎刺激。使用采用BEL-1开发的样品制备技术，可以对可变形生物材料进行高对比度和高分辨率观察，没有任何困难或复杂技术的必要性。这种形态学信息和开发的技术可以有助于揭示生物材料和试剂之间的相互作用，从而加速药物配制和疾病治疗。© 2017 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，106 B：1280-1285，2018。
In the medical biology, it is essential to understand not only biological morphology but also the interaction between biological materials and agents. To study these, electron microscopy (EM) is often utilized. However, sample preparation techniques for EM require a high level of skill and a considerable time. Here, we conducted EM using a simple technique employing a conductive liquid, BEL‐1, and compared the results with another simple technique employing an ionic liquid, 1‐butyl‐3‐methylimidazolium tetrafluoroborate ([BMIM][BF4]). BEL‐1 was used for sample pretreatment, and the morphologies of the mouse RAW 264.7 cell line,Porphyromonas gingivalis, and the RAW 264.7 cell line were stimulated via co‐incubation withP. gingivalisand observed using field emission scanning EM (FE‐SEM). In the present study, the inflammation‐induced system ofP. gingivaliswas successfully established. FE‐SEM results revealed the fine morphology of the RAW 264.7 cell line andP. gingivalisand confirmed a morphological change in the RAW 264.7 cell line caused byP. gingivalisstimulation. Using the developed sample preparation technique employing BEL‐1, high‐contrast and high‐resolution observations of deformable biological materials were conducted without any difficulty or the necessity for complicated technique. This morphological information and the developed techniques can contribute to reveal the interaction between biological materials and agents and thereby accelerate drug formulation and disease treatment. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1280–1285, 2018.