Identification of Cancer Stem Cells Derived From a Canine Lung Adenocarcinoma Cell Line
Identification of Cancer Stem Cells Derived From a Canine Lung Adenocarcinoma Cell Line
复制标题
DOI：
10.1177/0300985810396106
复制
发表时间：
2011-09-01
影响因子：
2.4
通讯作者：
Sahara, H.
中科院分区：
文献类型：
--
作者：
Nemoto, Y.;Maruo, T.;Sahara, H.
支持癌症干细胞（CSC）假说的证据越来越多，这是基于以下发现：肿瘤含有一小部分自我更新的细胞，这些细胞产生分化的后代，从而导致肿瘤异质性。据报道，CSC存在于几种人类癌症中，但只有少数报道证明CSC存在于犬的原发性肺癌中。在这项研究中，作者建立了一个来自犬原发性肺腺癌的癌细胞系，并确定了一个显示耐药特征的细胞侧群（SP）。为了证实这些SP细胞的特性，作者通过使用裸鼠异种移植模型研究了这些细胞在体内的致瘤性。只有100个SP细胞能够产生新的肿瘤，比主要细胞群（MP）富集10倍，表明这些细胞具有CSC的癌症引发能力。进一步研究犬肺腺癌中CSC的特征可能有助于阐明肿瘤发生的机制和建立新的治疗策略。
Accumulating evidence supporting the cancer stem cell (CSC) hypothesis is based on the finding that tumors contain a small population of self-renewing cells that generate differentiated progeny and thereby contribute to tumor heterogeneity. CSCs are reported to exist in several human cancers, yet only a few reports demonstrate the existence of CSCs in primary lung cancer in dogs. In this study, the authors established a cancer cell line derived from a canine primary lung adenocarcinoma and identified a side population (SP) of cells that displayed drug-resistant features. To confirm the characteristics of these SP cells, the authors investigated the tumorigenicity of the cells in vivo by using a nude mouse xenograft model. Only 100 SP cells were able to give rise to new tumors, giving a 10-fold enrichment over the main population (MP) of cells, suggesting that these cells have the cancer-initiating ability of CSCs. Further studies characterizing CSCs in canine lung adenocarcinoma might contribute to the elucidation of the mechanisms of tumorigenesis and to the establishment of novel therapeutic strategies.