INVIVO VISION OF THE HUMAN SKIN WITH THE TANDEM SCANNING MICROSCOPE
INVIVO VISION OF THE HUMAN SKIN WITH THE TANDEM SCANNING MICROSCOPE
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DOI：
10.1159/000247302
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发表时间：
1993-01-01
期刊：
影响因子：
3.4
通讯作者：
LEVEQUE, JL
中科院分区：
文献类型：
--
作者：
CORCUFF, P;LEVEQUE, JL
Petran 和 Hadravsky 发明的串联扫描反射光显微镜可在共焦模式下提供实时视觉。 它对生物材料进行无损光学切片，切片深度取决于组织的透明度。这个实时设施为活体标本的研究增加了第四个维度（时间）。在目前的工作中，我们在细胞水平上对人体皮肤进行了活体探索，深度可达 150 微米。研究了两个不同的解剖部位：手背和前臂掌侧。角质层的分层、整个表皮的活角质形成细胞的细胞核以及真皮浅层内的毛细血管袢构成了清晰的参考点。这些使得角质层和表皮的厚度测量精度达到 1 微米。这种探索方法为皮肤科医生和美容师提供了新的挑战。
The tandem scanning reflected light microscope invented by Petran and Hadravsky provides real-time vision in the confocal mode. It performs nondestructive optical sectioning of biological material to a depth that depends on the transparency of the tissue. This real-time facility adds a fourth dimension (time) to the study of living specimens. In the present work, human skin was explored in vivo, at the cellular level to a depth of 150 mum. Two different anatomical sites were investigated: the back of the hand and the volar aspect of the forearm. The stratification of the horny layers, the nuclei of the living keratinocytes through the whole epidermis and the capillary loops within the superficial dermis constituted clear reference points. These permitted thickness measurements of the stratum corneum and epidermis to an accuracy of 1 mum. This method of exploration provides a new challenge for dermatologists and cosmetologists.