The capsid protein of infectious bursal disease virus contains a functional α4β1 integrin ligand motif
The capsid protein of infectious bursal disease virus contains a functional α4β1 integrin ligand motif
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DOI：
10.1016/j.virol.2008.12.036
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发表时间：
2009-04-10
期刊：
影响因子：
3.7
通讯作者：
Rodriguez, Jose F.
中科院分区：
文献类型：
--
作者：
Delgui, Laura;Ona, Ana;Rodriguez, Jose F.
传染性法氏囊病病毒（IBDV）是双链RNA双核糖核酸病毒科（dsRNA Birnaviridae）的成员，是一种重要的免疫抑制性禽类病原体。我们已经鉴定了一个严格保守的氨基酸三联体，其与纤连蛋白用于结合IBDV衣壳多肽的突出结构域内的α 4 β 1整联蛋白的共有序列相匹配。我们发现，在这个三联体上的一个点突变废除了IBDV衍生的亚病毒颗粒（SVP）的细胞结合活性，并废除了通过反向遗传学回收传染性IBDV，而不影响整个SVP结构。此外，我们证明α 4 β 1异源二聚体的存在是小鼠BALB/c 3 T3细胞对IBDV结合和感染性敏感性的关键决定因素。我们的数据表明，IBDV也可能使用α 4 β 1整合素作为禽类细胞中的特异性结合受体。(C)2008年爱思唯尔公司All rights reserved.
Infectious bursal disease virus (IBDV), a member of the dsRNA Birnaviridae family, is an important immunosuppressive avian pathogen. We have identified a Strictly conserved amino acid triplet matching the consensus sequence used by fibronectin to bind the alpha 4 beta 1 integrin within the protruding domain of the IBDV capsid polypeptide. We show that a single point mutation on this triplet abolishes the cell-binding activity of IBDV-derived subviral particles (SVP), and abrogates the recovering of infectious IBDV by reverse genetics Without affecting the overall SVP architecture. Additionally, we demonstrate that the presence of the alpha 4 beta 1 heterodimer is a critical determinant for the Susceptibility Of murine BALB/c 3T3 cells to IBDV binding and infectivity. Our data suggests that the IBDV might also use the alpha 4 beta 1 integrin as a specific binding receptor in avian cells. (C) 2008 Elsevier Inc. All rights reserved.