Neutrophil depletion inhibits airway hyperresponsiveness induced by ozone exposure.
Neutrophil depletion inhibits airway hyperresponsiveness induced by ozone exposure.
复制标题
中性粒细胞消耗可抑制臭氧暴露引起的气道高反应性。
DOI：
10.1164/arrd.1984.130.2.214
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Holtzman,MJ
中科院分区：
文献类型：
--
作者：
O'Byrne,PM;Walters,EH;Gold,BD;Aizawa,HA;Fabbri,LM;Alpert,SE;Nadel,JA;Holtzman,MJ
我们研究了是否臭氧诱导的hyperresponsivenes可以抑制狗的中性粒细胞耗竭。通过乙酰胆碱气雾剂与肺阻力的剂量-反应曲线评估反应性;通过计数静脉血和气道上皮活检中的中性粒细胞来评估消耗。在6只未治疗的狗和6只用羟基脲治疗的狗（从臭氧前5天开始，每天200 mg/kg，持续5天）中，在臭氧前5天和臭氧后1小时（3.0 ppm，2小时）测定反应性和中性粒细胞数量。在未经处理的狗，反应性和中性粒细胞数臭氧前5天和1天没有改变，但反应性和上皮中性粒细胞臭氧后显着增加。在治疗犬中，循环中性粒细胞从8.9 ± 2.2减少到0.6 ± 0.01 × 103/mm 3（平均值± SEM），臭氧前的反应性没有变化。此外，增加反应性和上皮中性粒细胞没有发生臭氧后。停用羟基脲后6周，臭氧处理后反应性和上皮中性粒细胞明显增加。结果表明，臭氧引起的高反应性可能取决于动员中性粒细胞进入气道。
We studied whether ozone-induced hyperresponsivenes could be inhibited by neutrophil depletion in dogs. Responsiveness was assessed with dose-response curves of acetylcholine aerosol versus pulmonary resistance; depletion was assessed by counting neutrophils in venous blood and in biopsies of the airway epithelium. Responsiveness and neutrophil numbers were determined 5 days and 1 day before ozone and 1 h after ozone (3.0 ppm, 2 h) in 6 untreated dogs and in 6 dogs treated with hydroxyurea (200 mg/kg daily for 5 days starting 5 days before ozone). In untreated dogs, responsiveness and neutrophil numbers 5 days and 1 day before ozone did not change, but responsiveness and epithelial neutrophils increased markedly after ozone. In treated dogs, circulating neutrophils decreased from 8.9 ± 2.2 to 0.6 ± 0.01 x 103per mm3(mean ± SEM), and responsiveness before ozone did not change. Furthermore, increases in responsiveness and epithelial neutrophils did not occur after ozone. Six wk after stopping hydroxyurea, responsiveness and epithelial neutrophils increased markedly after ozone. The results suggest that ozone-induced hyperresponsiveness may depend on the mobilization of neutrophils into the airways.