Changes in Oxygen Level Upon Cold Plasma Treatments: Consequences for RONS Production
Changes in Oxygen Level Upon Cold Plasma Treatments: Consequences for RONS Production
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DOI：
10.1109/trpms.2017.2775705
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发表时间：
2018-03-01
影响因子：
4.4
通讯作者：
Grillon, Catherine
中科院分区：
文献类型：
--
作者：
Busco, Giovanni;Fasani, Fabienne;Grillon, Catherine
尽管大量的论文显示了冷大气等离子体（CAP）对皮肤治疗的生物学效应，并且尽管它们产生活性氧和氮物质（RONS）的混合物的能力在很大程度上是已知的，但是这些电离气体源的深入知识远未被发现。等离子体诱导的机理复杂，涉及到电压、频率、进气流量、温度、空气湿度、大气成分等诸多参数。在本文中，我们专注于我们的注意力在CAP处理过程中RONS产生的影响，溶解在培养基中的氧气。我们评估了在用两种不同百分比的氧平衡的Dulbecco磷酸盐缓冲盐水中RONS的产生：18%和3%O-2。前一种氧浓度通常用于大多数细胞培养和治疗（常氧），而后者接近人体皮肤内测量的生理水平（生理氧）。CAP处理后细胞内RONS的产生也在两个人皮肤细胞系中进行了测量，这两个细胞系保持在常氧或生理氧化条件下。我们的数据表明，RONS产生诱导的血浆中或细胞内的影响溶解在处理的目标的氧气。
Despite the large number of papers showing the biological effect of cold atmospheric plasmas (CAPs) on skin treatment and although their ability to produce a cocktail of reactive oxygen and nitrogen species (RONS) is largely known, the deep knowledge of these ionized gas sources is far to be uncovered. Many parameters take part in the complex mechanisms induced by plasma, such as voltage, frequency, feeding gas flow rate, temperature, air humidity, and atmospheric gas composition. In this paper, we focus our attention on the influence of oxygen dissolved in the medium in RONS generation during CAP treatment. We evaluate RONS production in Dulbecco's phosphate- buffered saline equilibrated with two different percentages of oxygen: 18% and 3% O-2. The former oxygen concentration is that usually used in most of cell culture and treatment (normoxia) while the latter is close to the physiological level measured inside human skin (physioxia). Intracellular RONS generation upon CAP treatment was also measured in two human skin cell lines maintained either in normoxic or in physioxic conditions. Our data suggest that RONS generation induced by plasma either in the medium or inside cells is influenced by oxygen dissolved in the treated target.