Gold nanoparticle-based colorimetric aptasensor for rapid detection of six organophosphorous pesticides
Gold nanoparticle-based colorimetric aptasensor for rapid detection of six organophosphorous pesticides
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DOI：
10.1002/etc.3088
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发表时间：
2015-10-01
影响因子：
4.1
通讯作者：
Chen, Ailiang
中科院分区：
文献类型：
--
作者：
Bai, Wenhui;Zhu, Chao;Chen, Ailiang
由于缺乏免疫原性和难以通过动物免疫获得抗体，基于免疫分析的快速筛选方法不适用于大多数小分子农药。适配子是通过体外过程筛选出的单链DNA分子，可以与任何靶点结合，包括高亲和力和高特异性的非免疫原性小分子。尽管基于适体的抗生素、微生物、重金属离子和生物毒素的传感方法已经开发出来，但基于适体的快速检测有机磷农药的方法还很少有报道。金(金)纳米颗粒(AuNP)比色法具有操作简单、结果直观等特点，是一种应用广泛的快速检测方法。在本研究中，有机磷农药适配子被吸附在AuNPs的表面，以稳定AuNP溶液对抗高浓度盐的聚集，以防止AuNP聚集。在添加靶标后，与靶标结合的适体从AuNPs中分离出来，导致AuNPs聚集，颜色从红色变为紫蓝色。该方法可用于环境河水中6种有机磷农药的检测，回收率在72%~135%之间。本研究为有机磷农药的简便、快速、多重检测提供了一种新的方法。环境毒物化学2015；34：2244-2249。(C)2015年SETAC
Fast immunoassay-based screening methods are unavailable for most small-molecule pesticides because of a lack of immunogenicity and the difficulty in obtaining antibodies by animal immunization. Aptamers are single-stranded DNA molecules selected through an in vitro process, which can bind to any target including nonimmunogenic small molecules with high affinity and specificity. Although various aptamer-based sensing methods have been developed for antibiotics, microorganisms, heavy metal ions, and biotoxins, there are few reports on aptamer-based methods for quick detection of organophosphorous pesticides. The gold (Au) nanoparticle (AuNP) colorimetric assay is a widely utilized rapid detection method because of properties such as easy operation and visualized results. In the present study, organophosphorous pesticide aptamers were adsorbed on the surface of AuNPs to stabilize the AuNP solution against high concentrations of salt to prevent AuNP aggregation. After the addition of targets, the aptamers binding to the targets are detached from the AuNPs, resulting in aggregation of AuNPs and a color change from red to purple-blue. The proposed method can detect 6 organophosphorous pesticides with good recoveries from 72% to 135% in environmental river water samples. The present study provides a new way for simple, rapid, and multiplex detection of organophosphorous pesticides. Environ Toxicol Chem 2015;34:2244-2249. (c) 2015 SETAC