H3.1K27me1 loss confers Arabidopsis resistance to Geminivirus by sequestering DNA repair proteins onto host genome.
H3.1K27me1 loss confers Arabidopsis resistance to Geminivirus by sequestering DNA repair proteins onto host genome.
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DOI：
10.1038/s41467-023-43311-1
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发表时间：
2023-11-18
影响因子：
16.6
通讯作者：
Zhang, Xiuren
中科院分区：
文献类型：
--
作者：
Wang, Zhen;Castillo-Gonzalez, Claudia M.;Zhao, Changjiang;Tong, Chun-Yip;Li, Changhao;Zhong, Songxiao;Liu, Zhiyang;Xie, Kaili;Zhu, Jiaying;Wu, Zhongshou;Peng, Xu;Jacob, Yannick;Michaels, Scott D.;Jacobsen, Steven E.;Zhang, Xiuren
H3甲基转移酶ATXR5和ATXR6将H3.1K27me1沉积到异染色质上，以防止基因组不稳定和转座子再激活。在这里，我们报道了atxr5 atxr6突变体对双病毒表现出强大的抗性。病毒抗性与DNA修复途径的激活有关，而与转座子再激活或异染色质扩增无关。我们发现RAD51和RPA1A是病毒编码Rep蛋白的伙伴。与野生型植物相比，这两种DNA修复蛋白在atxr5和atxr6中与异色区和防御相关基因的结合增加。因此，突变体中的蛋白质与病毒DNA的结合减少，从而阻碍了病毒的扩增。此外，RAD51通过BRCA1、HOP2和CYCB1募集到宿主基因组；而这种招募对于atxr5和atxr6的病毒耐药性至关重要。因此，双病毒通过劫持DNA修复途径来适应健康植物，而由还原H3.1K27me1触发的不稳定基因组可以保留DNA修复蛋白来抑制病毒在atxr5 atxr6中的扩增。双病毒劫持宿主DNA修复蛋白进行扩增。这组作者报告说，拟南芥失去了基因组稳定性保护因子H3.1K27me1，但通过保留RAD51等关键因子获得了对双病毒感染的抵抗力。
The H3 methyltransferases ATXR5 and ATXR6 deposit H3.1K27me1 to heterochromatin to prevent genomic instability and transposon re-activation. Here, we report that atxr5 atxr6 mutants display robust resistance to Geminivirus. The viral resistance is correlated with activation of DNA repair pathways, but not with transposon re-activation or heterochromatin amplification. We identify RAD51 and RPA1A as partners of virus-encoded Rep protein. The two DNA repair proteins show increased binding to heterochromatic regions and defense-related genes in atxr5 atxr6 vs wild-type plants. Consequently, the proteins have reduced binding to viral DNA in the mutant, thus hampering viral amplification. Additionally, RAD51 recruitment to the host genome arise via BRCA1, HOP2, and CYCB1;1, and this recruitment is essential for viral resistance in atxr5 atxr6. Thus, Geminiviruses adapt to healthy plants by hijacking DNA repair pathways, whereas the unstable genome, triggered by reduced H3.1K27me1, could retain DNA repairing proteins to suppress viral amplification in atxr5 atxr6. Geminiviruses hijack the host DNA repairing proteins for their amplification. The authors report that Arabidopsis loses H3.1K27me1, a protector of genome stability, but gains resistance to geminivirus infection via retaining key factors like RAD51.
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影响因子：
48
作者：
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通讯作者：
Salzberg, Steven L.
影响因子：
2
作者：
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影响因子：
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作者：
Bagewadi, B;Chen, SJ;Mukherjee, SK
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Mukherjee, SK
减数分裂中 RPA、RAD51 和 DMC1 结合的构型揭示了关键重组中间体的本质
影响因子：
16
作者：
Hinch, Anjali Gupta;Becker, Philipp W.;Donnelly, Peter
通讯作者：
Donnelly, Peter
DOI：
10.1093/plcell/koaa027
发表时间：
2021-05-31
期刊：
The Plant cell
影响因子：
--
作者：
Dong J;LeBlanc C;Poulet A;Mermaz B;Villarino G;Webb KM;Joly V;Mendez J;Voigt P;Jacob Y
通讯作者：
Jacob Y