Introduction of vasculature in engineered three-dimensional tissue.
Introduction of vasculature in engineered three-dimensional tissue.
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DOI：
10.1186/s41232-017-0055-4
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发表时间：
2017
影响因子：
8.1
通讯作者：
Shimizu T
中科院分区：
文献类型：
--
作者：
Sekiya S;Shimizu T
随着组织工程技术的发展，现在可以生成各种三维组织。然而，随着组织厚度由于三维化而增加，在不引入血管的情况下难以增加组织规模。近年来，已有许多血管诱导方法的报道。本文从三个方面介绍了几种在三维组织中实现可调血管化的方法。首先，“选择”为工程化组织提供了血管化能力。第二，“组装技术”用于将组织制造为三维结构，同时制造内部新血管。第三，“灌注”技术用于三维组织中血管的成熟。在“选择”中，对细胞和材料的选择赋予在三维组织中促进血管生成的能力。在细胞组装步骤中，可以使用细胞片工程、纳米膜涂层技术和三维打印技术来生产血管化三维组织。通过统一的入口和出口在整个三维组织中灌注血液或细胞培养基的灌注技术可以在可再移植的三维组织内诱导功能性血管。每一步技术的组合允许模拟靶组织中的血管周围微环境并驱动三维组织中的血管化。靶组织的仿生微环境将诱导足够的细胞-细胞相互作用、距离、细胞形态和组织内的功能。它可以加速三维组织内的血管化，并为我们提供功能组织。由于血管化三维组织具有高度功能性，因此预计它们将有助于未来再生医学和药物安全性测试的发展。
With recent developments in tissue engineering technology, various three-dimensional tissues can be generated now. However, as the tissue thickness increases due to three-dimensionalization, it is difficult to increase the tissue scale without introduction of blood vessels. Many methods for vasculature induction have been reported recently. In this review, we introduced several methods which are adjustable vascularization in three-dimensional tissues according to three steps. First, “selection” provides potents for engineered tissues with vascularization ability. Second, “assembly technology” is used to fabricate tissues as three-dimensional structures and simultaneously inner neo-vasculature. Third, a “perfusion” technique is used for maturation of blood vessels in three-dimensional tissues. In “selection”, selection of cells and materials gives the ability to promote angiogenesis in three-dimensional tissues. During the cell assembly step, cell sheet engineering, nanofilm coating technology, and three-dimensional printing technology could be used to produce vascularized three-dimensional tissues. Perfusion techniques to perfuse blood or cell culture medium throughout three-dimensional tissues with a unified inlet and outlet could induce functional blood vessels within retransplantable three-dimensional tissues. Combination of each step technology allows simulation of perivascular microenvironments in target tissues and drive vascularization in three-dimensional tissues. The biomimetic microenvironment of target tissues will induce adequate cell-cell interaction, distance, cell morphology, and function within tissues. It could be accelerated for vascularization within three-dimensional tissues and give us the functional tissues. Since vascularized three-dimensional tissues are highly functional, they are expected to contribute to the development of regenerative medicine and drug safety tests for drug discovery in the future.