SPECIFIC DNA METHYLATION SITES IN THE VICINITY OF THE CHICKEN BETA-GLOBIN GENES
SPECIFIC DNA METHYLATION SITES IN THE VICINITY OF THE CHICKEN BETA-GLOBIN GENES
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DOI：
10.1038/280419a0
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发表时间：
1979-01-01
期刊：
影响因子：
64.8
通讯作者：
GINDER, GD
中科院分区：
文献类型：
--
作者：
MCGHEE, JD;GINDER, GD
胞嘧啶甲基化是迄今为止在高等真核生物DNA中检测到的唯一一种合成后修饰，因此已被作为几种已提出的基因活性和细胞分化机制的基础1-3。然而，所有证据表明，DNA中5-甲基胞嘧啶的总含量在同一生物的不同组织之间没有显著差异，至少在分化的某些步骤4-6中没有变化。由于许多细菌限制性内切酶可以区分该序列是否甲基化，现在可以研究单基因附近的DNA甲基化模式8。在这份报告中，我们使用这项技术来提供DNA甲基化和鸡β-珠蛋白基因活性之间的关系。
METHYLATION of cytosine is the only post-synthetic modification so far detected in the DNA of higher eukaryotes and thus has been made the basis of several proposed mechanisms of gene activity and cellular differentiation1–3. All evidence indicates, however, that the overall content of 5-methylcytosine in DNA does not vary significantly between different tissues of the same organism and does not change throughout at least some steps of differentiation4–6. As 5-methylcytosine occurs predominantly in the dinucleotide sequence CpG (ref. 7) and as a number of bacterial restriction endonucleases can distinguish whether this sequence is methylated or unmethylated, it is now possible to investigate DNA methylation patterns in the vicinity of single genes8. In this report, we use this technique to provide a correlation between DNA methylation and the activity of the chickenβ-globin genes.