CDR3-independent expansion of Vδ1 T lymphocytes in acquired chronic pure red cell aplasia
CDR3-independent expansion of Vδ1 T lymphocytes in acquired chronic pure red cell aplasia
复制标题
DOI：
10.1016/j.imlet.2012.12.006
复制
发表时间：
2013-02-01
影响因子：
4.4
通讯作者：
Sawada, Kenichi
中科院分区：
文献类型：
--
作者：
Michishita, Yoshihiro;Hirokawa, Makoto;Sawada, Kenichi
尽管有病例报告描述了 γδT 细胞的克隆扩增与慢性获得性纯红细胞再生障碍性贫血 (PRCA) 之间的关联，但对于慢性获得性纯红细胞再生障碍性贫血 (PRCA) 中是否普遍存在 γδT 细胞的克隆扩增尚未达成共识。我们检查了 19 名 PRCA 患者的 γ δ T 细胞受体库，发现 PRCA 患者和健康供体之间的 γ δ T 细胞受体库存在差异。我们观察到 PRCA 患者中 V δ 1 γ δ T 细胞增加，V δ 2 T 细胞减少。在所检查的 13 名 PRCA 患者中，有 9 名的 CDR3 delta 1 大小分布模式存在偏差，尽管在 10 名健康个体中有 7 名也观察到这种偏差。 PRCA 患者和健康供体之间的 CDR3 delta 1 大小分布没有显着变化。此外，在 PRCA 患者中没有发现明显的共有氨基酸基序。 V delta 1 T 细胞的扩增和 V delta 2 T 细胞的耗竭是慢性获得性 PRCA 的独特特征，但 V delta 1 T 细胞的扩增似乎并不是 CDR3 依赖性选择的结果。我们得出的结论是，V delta 1 T 细胞的克隆扩增并不是慢性获得性 PRCA 的普遍特征。 (c) 2012 Elsevier B.V. 保留所有权利。
Although there exist case reports describing the association of clonal expansion of gamma delta T cells with chronic acquired pure red cell aplasia (PRCA), there is no consensus regarding whether clonal expansion of gamma delta T cells are generally found in chronic PRCA. We examined the gamma delta T cell receptor repertoire in 19 PRCA patients and found that there was a difference in gamma delta T-cell repertoires between PRCA patients and healthy donors. We observed an increase in V delta 1 gamma delta T cells and a decrease in V delta 2 T cells in PRCA patients. CDR3 delta 1 size distribution patterns were skewed in 9 out of 13 PRCA patients examined, although the skewing was also observed in 7 out of 10 healthy individuals. No significant changes were present in CDR3 delta 1 size distribution between PRCA patients and healthy donors. Moreover, no apparent consensus amino acid motifs were identified in PRCA patients. Expansion of V delta 1 T cells and depletion of V delta 2 T cells are unique features for chronic acquired PRCA but expansion of V delta 1 T cells does not seem to be the consequence of CDR3-dependent selection. We conclude that clonal expansion of V delta 1 T cells is not a general feature for chronic acquired PRCA. (c) 2012 Elsevier B.V. All rights reserved.