Contiguous RNA editing sites in the mitochondrial nad1 transcript of Arabidopsis thaliana are recognized by different proteins
Contiguous RNA editing sites in the mitochondrial nad1 transcript of Arabidopsis thaliana are recognized by different proteins
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DOI：
10.1016/j.febslet.2013.02.009
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发表时间：
2013-04-02
期刊：
影响因子：
3.5
通讯作者：
Jordana, Xavier
中科院分区：
文献类型：
--
作者：
Arenas-M, Anita;Takenaka, Mizuki;Jordana, Xavier
五肽重复（PPR）蛋白已被确定为植物细胞器中RNA编辑的位点特异性因子。这些蛋白质识别编辑位点附近的顺式元件。目前还不清楚如何确定相邻的地点，以及是否需要一个或两个因素。我们在这里证明了PPR MEF25对于拟南芥线粒体nad1-308位点的RNA编辑是必不可少的。另一个编辑位点nad1-307位于mef25突变系上游，在mef25突变系中被正常编辑。这一发现表明，识别相似顺式元件的两个独立因子参与了这些连续位点，而在体内彼此不竞争。(C) 2013年欧洲生化学会联合会。Elsevier b.v.版权所有。
Pentatricopeptide repeat (PPR) proteins have been identified as site-specific factors for RNA editing in plant organelles. These proteins recognize cis-elements near the editing site. It is unclear how contiguous sites are addressed, and whether one or two factors are required. We here show the PPR MEF25 to be essential for RNA editing at the nad1-308 site in Arabidopsis mitochondria. Another editing site just one nucleotide upstream, nad1-307, is edited normally in mef25 mutant lines. This finding shows that two independent factors recognizing similar cis-elements are involved at these contiguous sites without competing with each other in vivo. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.