Control of Plant Viruses by CRISPR/Cas System-Mediated Adaptive Immunity.

Control of Plant Viruses by CRISPR/Cas System-Mediated Adaptive Immunity.
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通过 CRISPR/Cas 系统介导的适应性免疫控制植物病毒。

DOI:
10.3389/fmicb.2020.593700
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发表时间:
2020
影响因子:
5.2
通讯作者:
Li F
Li F
中科院分区:
生物学2区
文献类型:
--
作者:
Cao Y;Zhou H;Zhou X;Li F

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由入侵植物病毒引起的植物病害对世界农业生产构成严重威胁,由分子生物技术引发的抗病毒工程已成为防治植物病毒的有效策略。在植物中成簇的规则间隔短回文重复序列(CRISPR)/CRISPR相关(Cas)系统介导的DNA或RNA编辑/干扰的最新进展使它们成为适用于植物保护领域的非常有吸引力的工具。本文综述了CRISPR/Cas系统的发展,并总结了其在通过靶向病毒序列或宿主易感基因控制不同植物病毒方面的应用。我们列出了一些潜在的隐性抗性基因,可用于抗病毒育种,并强调了隐性抗性基因为基础的抗病毒育种的重要性和承诺,以产生无发育缺陷的无转基因植物。最后,我们讨论了CRISPR/Cas技术在植物病毒防控领域应用的挑战和机遇。
Plant diseases caused by invading plant viruses pose serious threats to agricultural production in the world, and the antiviral engineering initiated by molecular biotechnology has been an effective strategy to prevent and control plant viruses. Recent advances in clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system-mediated DNA or RNA editing/interference in plants make them very attractive tools applicable to the plant protection field. Here, we review the development of CRISPR/Cas systems and summarize their applications in controlling different plant viruses by targeting viral sequences or host susceptibility genes. We list some potential recessive resistance genes that can be utilized in antiviral breeding and emphasize the importance and promise of recessive resistance gene-based antiviral breeding to generate transgene-free plants without developmental defects. Finally, we discuss the challenges and opportunities for the application of CRISPR/Cas techniques in the prevention and control of plant viruses in the field.
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影响因子: 4.7
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