Effect of Torsion on Cisplatin-Induced DNA Condensation
Effect of Torsion on Cisplatin-Induced DNA Condensation
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扭转对顺铂诱导的 DNA 缩合的影响
DOI：
10.1088/0256-307x/35/11/118701
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发表时间：
2018
影响因子：
3.5
通讯作者：
Wang Peng-Ye
中科院分区：
文献类型：
--
作者：
Li Bo;Ji Chao;Lu Xi-Ming;Liu Yu-Ru;Li Wei;Dou Shuo-Xing;Li Hui;Wang Peng-Ye
利用原子力显微镜，以共价闭合的环形DNA为扭转约束体系，研究了扭转对DNA缩合的影响。研究发现，在扭转约束下，DNA缩合过程分为两个阶段。在早期，低扭转会通过促进微环或交叉结构的形成来加速DNA缩合；而在后期，由于DNA分子变得更加刚性，增加的扭转会阻止顺铂与DNA的交联，从而减缓DNA缩合。我们的结果显示了扭转在DNA缩合中的重要作用，并为揭示DNA缩合的机制提供了新的线索。
We investigate the effect of torsion on DNA condensation with the covalently closed circular DNA as the torsion-constrained system, using an atomic force microscope. It is found that there are two stages in the DNA condensation process under torsional constraint. At the early stage, the low torsion will accelerate DNA condensation by promoting the formation of micro-loops or intersection structures; while at the later stage, the increasing torsion will slow it down by preventing the crosslinking of cisplatin and DNA since the DNA molecule becomes more rigid. Our results show the important role of torsion in DNA condensation and sheds new light on the mechanism for DNA condensation.