Structural and Functional Dynamics of Sulfate-Reducing Populations in Bacterial Biofilms
Structural and Functional Dynamics of Sulfate-Reducing Populations in Bacterial Biofilms
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DOI：
10.1128/aem.64.10.3731-3739.1998
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发表时间：
1998-10
影响因子：
4.4
通讯作者：
C. M. Santegoeds;T. Ferdelman;G. Muyzer;D. de Beer
中科院分区：
文献类型：
--
作者：
C. M. Santegoeds;T. Ferdelman;G. Muyzer;D. de Beer
摘要我们描述了微传感器和分子技术的结合应用，以调查硫酸盐还原和硫酸盐还原菌群在好氧细菌生物膜的发展。氧的微传感器测量显示，厌氧区在1周内在生物膜中发展，并且在生物膜发展的所有阶段，氧在顶部200至400 μm中耗尽。硫酸盐还原是第一次检测后6周的增长，虽然有利的条件下生长的硫酸盐还原菌（SRB）从第一周。SRB的16 S rRNA探针原位杂交显示，硫酸盐还原剂存在于高的数字（约108 SRB/ml）在所有发展阶段，无论是在好氧和缺氧区的生物膜。变性梯度凝胶电泳（DGGE）结果表明，随着生物膜的形成，微生物群落的遗传多样性逐渐增加。DGGE图谱与分类群特异性寡核苷酸探针的杂交分析表明，脱硫弧菌和脱硫弧菌是所有生物膜样品以及散装活性污泥中的主要硫酸盐还原菌。然而，在培养的第6周和第8周，分别发现了不同的脱硫弧菌和脱硫弧菌，与硫酸盐还原的发展相一致。我们的数据表明，并不是所有的SRB分子分析检测到的生物膜中的硫化物活性。
ABSTRACT We describe the combined application of microsensors and molecular techniques to investigate the development of sulfate reduction and of sulfate-reducing bacterial populations in an aerobic bacterial biofilm. Microsensor measurements for oxygen showed that anaerobic zones developed in the biofilm within 1 week and that oxygen was depleted in the top 200 to 400 μm during all stages of biofilm development. Sulfate reduction was first detected after 6 weeks of growth, although favorable conditions for growth of sulfate-reducing bacteria (SRB) were present from the first week. In situ hybridization with a 16S rRNA probe for SRB revealed that sulfate reducers were present in high numbers (approximately 108 SRB/ml) in all stages of development, both in the oxic and anoxic zones of the biofilm. Denaturing gradient gel electrophoresis (DGGE) showed that the genetic diversity of the microbial community increased during the development of the biofilm. Hybridization analysis of the DGGE profiles with taxon-specific oligonucleotide probes showed thatDesulfobulbus and Desulfovibrio were the main sulfate-reducing bacteria in all biofilm samples as well as in the bulk activated sludge. However, different Desulfobulbus andDesulfovibrio species were found in the 6th and 8th weeks of incubation, respectively, coinciding with the development of sulfate reduction. Our data indicate that not all SRB detected by molecular analysis were sulfidogenically active in the biofilm.