Mechanical behavior of metastatic vertebrae are influenced by tissue architecture, mineral content, and organic feature alterations
Mechanical behavior of metastatic vertebrae are influenced by tissue architecture, mineral content, and organic feature alterations
复制标题
DOI：
10.1002/jor.24105
复制
发表时间：
2018-11-01
影响因子：
2.8
通讯作者：
Whyne, Cari
中科院分区：
文献类型：
--
作者：
Burke, Mikhail;Akens, Margarete;Whyne, Cari
转移性病变的椎体力学性能下降通常归因于结构和骨小梁含量的改变。以前的工作已经确定了有机和矿物质相骨质量的变化，在存在转移，但有限的工作存在的潜在影响，这种组织水平的修改对椎骨的机械特性。这项工作旨在确定骨（结构和组织水平）的功能和转移涉及椎骨的力学行为之间的相关性。据推测，组织水平的属性（矿物和有机）将改善这些相关性超出建筑性能和BMD单独。24只雌性无胸腺大鼠分别接种产生溶骨性（N = 8）或混合性（溶骨性/成骨细胞，N = 7）转移的HeLa或Ace-1癌细胞系。接种后21天，切除L1-L3病理性椎骨运动节段并进行mu CT成像。对L2椎体的三维形态测量参数和轴向刚度进行量化。连续加载和mu CT成像测量了失效、刚度和峰值力的进展。利用线性回归模型评价了机械测试（全骨和组织水平）和组织水平材料性质改变与转移性累及之间的关系。与健康对照组（N = 9）相比，溶骨性病变降低了椎体松质骨体积、结构、CT轴向刚度、刚度和失效力。混合转移显示出类似的趋势。先前评估的胶原交联和脯氨酸基残基与机械行为相关，并提高了回归模型的预测能力。同样，胶原组织改善了转移性骨硬度的预测回归模型。这项工作突出了骨含量/结构和有机组织水平的特点，转移性椎体力学的重要性。(c)2018骨科研究学会。由威利期刊公司出版J Orthop Res 36：3013-3022，2018。
Diminished vertebral mechanical behavior with metastatic involvement is typically attributed to modified architecture and trabecular bone content. Previous work has identified organic and mineral phase bone quality changes in the presence of metastases, yet limited work exists on the potential influence of such tissue level modifications on vertebral mechanical characteristics. This work seeks to determine correlations between features of bone (structural and tissue level) and mechanical behavior in metastatically involved vertebral bone. It is hypothesized that tissue level properties (mineral and organic) will improve these correlations beyond architectural properties and BMD alone. Twenty-four female athymic rats were inoculated with HeLa or Ace-1 cancer cells lines producing osteolytic (N = 8) or mixed (osteolytic/osteoblastic, N = 7) metastases, respectively. Twenty-one days post-inoculation L1-L3 pathologic vertebral motion segments were excised and mu CT imaged. 3D morphometric parameters and axial rigidity of the L2 vertebrae were quantified. Sequential loading and mu CT imaging measured progression of failure, stiffness and peak force. Relationships between mechanical testing (whole bone and tissue-level) and tissue-level material property modifications with metastatic involvement were evaluated utilizing linear regression models. Osteolytic involvement reduced vertebral trabecular bone volume, structure, CT-derived axial rigidity, stiffness and failure force compared to healthy controls (N = 9). Mixed metastases demonstrated similar trends. Previously assessed collagen cross-linking and proline-based residues were correlated to mechanical behavior and improved the predictive ability of the regression models. Similarly, collagen organization improved predictive regression models for metastatic bone hardness. This work highlights the importance of both bone content/architecture and organic tissue-level features in characterizing metastatic vertebral mechanics. (c) 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:3013-3022, 2018.