Identification of anti-α-amylase components from olive leaf extracts
Identification of anti-α-amylase components from olive leaf extracts
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DOI：
10.3136/fstr.9.35
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发表时间：
2003-02-01
影响因子：
0.6
通讯作者：
Tsukada, Y
中科院分区：
文献类型：
--
作者：
Komaki, E;Yamaguchi, S;Tsukada, Y
橄榄（Olea europaea L.）是欧洲公认的治疗糖尿病的民间药物。研究了橄榄叶乙醇提取物（OEE）对人淀粉酶活性的体外抑制作用。OEE对人唾液和胰腺α -淀粉酶活性有抑制作用，IC50值分别为4.0和0.02 mg/ml。使用Sephadex LH-20柱层析从OEE的50%乙醇可溶性部分纯化了两种抗α -淀粉酶成分。一种鉴定为木犀草素-7- o - β -葡萄糖苷，另一种鉴定为木犀草素-4'- o - β -葡萄糖苷。将OEE的50%乙醇不溶部分溶解在98%乙醇中，用Cosmosil C18-OPN柱层析进行分离。经层析纯化的抗淀粉酶成分鉴定为齐墩果酸。木犀草素和齐墩果酸对糖尿病大鼠餐后血糖升高均有抑制作用。在临界志愿者（空腹血糖：110-140 mg/dl）口服淀粉后，橄榄叶抑制血糖升高，因此它们可能是一种有用的预防糖尿病的食物补充剂。
Olive (Olea europaea L.) is recognized as a folk medicine for diabetes in Europe. The inhibitory action of an ethanol extract of olive leaves (OEE) on the activities of human amylases was examined in vitro. OEE inhibited the activities of alpha-amylases from human saliva and pancreas with IC50 values of 4.0 and 0.02 mg/ml, respectively. Two anti-alpha-amylase components were purified from a 50% ethanol soluble fraction of OEE using Sephadex LH-20 column chromatography. One was identified as luteolin-7-O-beta glucoside and the other as luteolin-4'-O-beta glucoside. The 50% ethanol insoluble fraction of OEE was dissolved in 98% ethanol and fractionated using Cosmosil C18-OPN column chromatography. The anti-alpha-amylase component purified by this chromatography was identified as oleanolic acid. Both luteolin and oleanolic acid have an inhibitory effect on postprandial blood glucose increase in diabetic rats. Olive leaves suppressed the elevation of blood glucose after oral administration of starch in borderline volunteers (fasting blood glucose: 110-140 mg/dl), and thus they may be a useful food supplement for the prevention of diabetes.