Translocation of cAMP-dependent protein kinase to the nucleus during development of Dictyostelium discoideum.
Translocation of cAMP-dependent protein kinase to the nucleus during development of Dictyostelium discoideum.
复制标题
盘基网柄菌发育过程中 cAMP 依赖性蛋白激酶易位至细胞核。
DOI：
10.1016/0012-1606(86)90221-6
复制
发表时间：
1986
影响因子：
2.7
通讯作者：
Rickenberg,HV
中科院分区：
文献类型：
--
作者：
Woffendin,C;Chambers,TC;Schaller,KL;Leichtling,BH;Rickenberg,HV
在盘基网柄藻（Dictyosterichdiscoideum）发育过程中，cAMP依赖性蛋白激酶（cAMP-dependentproteinkinase，CPK）的催化亚基和调节亚基在细胞质和细胞核之间的分布被确定。在营养阿米巴中，大约2%的亚基位于细胞核中。在发育过程中，总可溶性cAMP依赖性蛋白激酶增加约5倍，细胞核部分酶增加15至30倍。当尖端聚集体被破坏并在单细胞悬浮液中孵育所得阿米巴时，存在从细胞核到细胞质的反向易位。向这些单细胞悬浮液中加入cAMP，使亚基重新进入细胞核。研究结果进行了讨论有关的cAMP依赖性蛋白激酶在mRNA和蛋白质合成的调节的潜在作用。
The distribution of the catalytic and regulatory subunits of the cAMP-dependent protein kinase between cytoplasm and nucleus was determined during the development ofDictyostelium discoideum. In vegetative amoebae approximately 2% of the subunits were in the nucleus. During development there was an approximately 5-fold increase in total soluble cAMP-dependent protein kinase and a 15- to 30-fold increase of enzyme in the nuclear fraction. There was a reverse translocation from nucleus to cytoplasm, when Tipped Aggregates were disrupted and the resultant amoebae incubated in single-cell suspension. The addition of cAMP to these single-cell suspensions brought about the reentry of the subunits into the nucleus. The findings are discussed in relation to the potential role of the cAMP-dependent protein kinase in the regulation of mRNA and protein synthesis.