The genes encoding the secreted aspartyl proteinases of Candida albicans constitute a family with at least three members.
The genes encoding the secreted aspartyl proteinases of Candida albicans constitute a family with at least three members.
复制标题
编码白色念珠菌分泌的天冬氨酰蛋白酶的基因构成一个具有至少三个成员的家族。
DOI：
10.1128/iai.61.8.3240-3243.1993
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发表时间：
1993
影响因子：
3.1
通讯作者：
Magee,PT
中科院分区：
文献类型：
--
作者：
Magee,BB;Hube,B;Wright,RJ;Sullivan,PJ;Magee,PT
白念珠菌分泌的β-羟丙基蛋白酶活性被认为是一种潜在的毒力因子。四个实验室已经从C.白色念珠菌编码这种酶。当这些基因中的两个在DNA水平上共享77%的同源性时，在高度严格的条件下与四种不同菌株的轮廓钳位均匀电场染色体分离杂交，它们标记不同的染色体：SAP 1的染色体6和SAP 2的染色体R。通过聚合酶链反应证实了这两个序列的不同基因的存在。用基因探测并在低严格条件下洗涤的基因组Southern印迹显示了几个交叉杂交条带。在低严格性下探测的轮廓钳位均匀电场染色体分离表明，除了染色体R和6上的序列外，3号染色体上还有一个交叉杂交序列。对C.因此，白色念珠菌构成了我们称之为SAP家族的基因家族。
The secreted aspartyl proteinase activity from Candida albicans is thought to be a potential virulence factor. Four laboratories have cloned a gene from C. albicans encoding this enzyme. When two of these genes sharing 77% homology at the DNA level are hybridized under conditions of high stringency to contour-clamped homogeneous electric field chromosome separations of four different strains, they label different chromosomes: chromosome 6 for SAP1 and chromosome R for SAP2. The existence of different genes for the two sequences was confirmed by polymerase chain reaction. Genomic Southern blots probed with the genes and washed at low stringency revealed several cross-hybridizing bands. Contour-clamped homogeneous electric field chromosome separations probed at low stringency indicated that there was a cross-hybridizing sequence on chromosome 3 in addition to those on chromosomes R and 6. The genes for the secreted aspartyl proteinase activity in C. albicans thus constitute a gene family which we have called the SAP family.