Labeling of fatty acids with 4-bromomethyl-7-methoxycoumarin via crown ether catalyst for fluorimetric detection in high-performance liquid chromatography
Labeling of fatty acids with 4-bromomethyl-7-methoxycoumarin via crown ether catalyst for fluorimetric detection in high-performance liquid chromatography
复制标题
通过冠醚催化剂用 4-溴甲基-7-甲氧基香豆素标记脂肪酸，用于高效液相色谱中的荧光检测
DOI：
10.1016/s0021-9673(00)89967-7
复制
发表时间：
1978
影响因子：
4.1
通讯作者：
E. Grushka
中科院分区：
文献类型：
--
作者：
S. Lam;E. Grushka
A method for the fluorescence labeling of fatty acids with 4-bromomethyl-7-methoxycoumarin using 18-crown-6 as a catalyst is described. The procedure is rapid, simple and requires little or no experience in derivatization techniques. The amounts of the derivatives are linearly related to the amount of the starting fatty acids and the procedure can therefore be used in the quantitative analysis of these solutes. Using reversed-phase chromatography, detection levels as low as 9 pmole could be realized. The separations of several derivatives using a reversed-phase system are discussed.
