Optimization of an Image-Guided Laser-Induced Choroidal Neovascularization Model in Mice.
Optimization of an Image-Guided Laser-Induced Choroidal Neovascularization Model in Mice.
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DOI：
10.1371/journal.pone.0132643
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Smith LE
中科院分区：
文献类型：
--
作者：
Gong Y;Li J;Sun Y;Fu Z;Liu CH;Evans L;Tian K;Saba N;Fredrick T;Morss P;Chen J;Smith LE
激光诱导的脉络膜新生血管（CNV）的小鼠模型已被用于研究渗出形式的年龄相关性黄斑变性，使用传统的裂隙灯和新的图像引导激光系统。需要一个标准化的协议，使用这个模型，这一直缺乏一致的结果。我们使用图像引导激光光凝系统优化了激光诱导CNV的细节。使用不同的激光功率水平和不同年龄和性别的小鼠，在距离视神经约两倍的椎间盘直径处，对每只眼睛持续施加四个大小相似的损伤。7天后，处死小鼠，将视网膜色素上皮/脉络膜/巩膜平铺，用异凝集素B4染色，并成像。使用确定的恒定阈值对激光诱导损伤的面积进行定量。排除标准的描述是必要的可靠的数据分析的激光诱导CNV病变。CNV损伤面积与激光功率水平成正比。12-16周龄的小鼠比6-8周龄的小鼠发生更严重的CNV，并且性别差异仅在12-16周龄的小鼠中显著，而在6-8周龄的小鼠中不显著。膳食摄入omega-3长链多不饱和脂肪酸可降低小鼠激光诱导的CNV。综上所述，使用图像引导激光平台可以容易且一致地应用激光诱导的CNV损伤。6-8周龄的小鼠是激光诱导的CNV模型的理想动物。
The mouse model of laser-induced choroidal neovascularization (CNV) has been used in studies of the exudative form of age-related macular degeneration using both the conventional slit lamp and a new image-guided laser system. A standardized protocol is needed for consistent results using this model, which has been lacking. We optimized details of laser-induced CNV using the image-guided laser photocoagulation system. Four lesions with similar size were consistently applied per eye at approximately double the disc diameter away from the optic nerve, using different laser power levels, and mice of various ages and genders. After 7 days, the mice were sacrificed and retinal pigment epithelium/choroid/sclera was flat-mounted, stained with Isolectin B4, and imaged. Quantification of the area of the laser-induced lesions was performed using an established and constant threshold. Exclusion criteria are described that were necessary for reliable data analysis of the laser-induced CNV lesions. The CNV lesion area was proportional to the laser power levels. Mice at 12-16 weeks of age developed more severe CNV than those at 6-8 weeks of age, and the gender difference was only significant in mice at 12-16 weeks of age, but not in those at 6-8 weeks of age. Dietary intake of omega-3 long-chain polyunsaturated fatty acid reduced laser-induced CNV in mice. Taken together, laser-induced CNV lesions can be easily and consistently applied using the image-guided laser platform. Mice at 6-8 weeks of age are ideal for the laser-induced CNV model.
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影响因子：
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