MOLECULAR-CLONING OF THE WHITE LOCUS REGION OF DROSOPHILA-MELANOGASTER USING A LARGE TRANSPOSABLE ELEMENT
MOLECULAR-CLONING OF THE WHITE LOCUS REGION OF DROSOPHILA-MELANOGASTER USING A LARGE TRANSPOSABLE ELEMENT
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DOI：
10.1002/j.1460-2075.1982.tb01130.x
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发表时间：
1982-01-01
期刊：
影响因子：
11.4
通讯作者：
GEHRING, WJ
中科院分区：
文献类型：
--
作者：
GOLDBERG, ML;PARO, R;GEHRING, WJ
报道了黑腹隐翅虫白色基因片段的分子克隆。该区域是利用从先前克隆的87A7的热休克喷雾序列延伸到包含白色和最粗略座位的大的转座元件的缺陷来分离的。FB-NOF是一个7.5kb的元件，与一组反向重复序列有部分同源性，在缺失断裂点附近被发现，紧随其后的是源自白色基因座的DNA。总计.apprx的序列。通过克隆野生型菌株的连续重叠片段，获得了该起始入口点周围60kb的片段。根据克隆的DNA定位了几个重排断裂点；这些断裂点定义了白色基因座的界限，并进一步将白色近端区域(被认为具有基因调控功能)与该基因座的其余部分区分开来。在携带白杏等位基因的品系中，观察到分散的重复元件CopiA插入到白色基因座中。对几个白杏返回体的分析表明，Copia插入是杏眼颜色表型的原因。
The molecular cloning of a chromosome segment including the white locus of D. melanogaster is reported. This region was isolated using a deficiency extending from the previously cloned heat-shock puff sequences at 87A7 to a large transposable element containing the loci white and roughest. FB-NOF, a 7.5 kb [kilobase] element with partial homology to a family of inverted repeat sequences, is found very near the deficiency breakpoint, and is followed by DNA originating from the white locus region. Sequences totalling .apprx. 60 kb surrounding this initial entry point were obtained by the cloning of successively overlapping fragments from a wild-type strain. Several rearrangement breakpoints were mapped relative to the cloned DNA; these define the limits of the white locus and further differentiate the white proximal region (thought to function in gene regulation) from the remainder of the locus. Insertion of the dispersed repetitive element copia into the white locus is observed in strains carrying the white-apricot allele. Analysis of several white-apricot revertants suggests that copia insertion is responsible for the apricot eye color phenotype.