SEPARATION OF THE COMPLEX ASPARAGINE-LINKED OLIGOSACCHARIDES OF THE GLYCOPROTEIN FETUIN AND ELUCIDATION OF 3 TRIANTENNARY STRUCTURES HAVING SIALIC ACIDS LINKED ONLY TO GALACTOSE RESIDUES
SEPARATION OF THE COMPLEX ASPARAGINE-LINKED OLIGOSACCHARIDES OF THE GLYCOPROTEIN FETUIN AND ELUCIDATION OF 3 TRIANTENNARY STRUCTURES HAVING SIALIC ACIDS LINKED ONLY TO GALACTOSE RESIDUES
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DOI：
10.1021/bi00441a050
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发表时间：
1989-07-25
期刊：
影响因子：
2.9
通讯作者：
CUMMING, DA
中科院分区：
文献类型：
--
作者：
BENDIAK, B;HARRISBRANDTS, M;CUMMING, DA
糖蛋白胎球蛋白的天冬酰胺连接的寡糖在链霉蛋白酶消化的糖蛋白的肼解/re-N-乙酰化/弱酸处理后作为还原性寡糖分离。通过高效液相色谱法在两个不同的系统中分离唾液酸化低聚糖，这导致大于35个级分，包括二-，三-，四-和五唾液酸化低聚糖。主要组分是包含三-和四唾液酸化级分的异构体结构。在这篇论文和所附的论文（Cumming等人，1989），描述了三-、四-和五唾液酸化低聚糖级分的10种主要组分的结构。本文提出了分离方案和三个分离的结构，唾液酸仅连接到半乳糖。
Asparagine-linked oligosaccharides of he glycoprotein fetuin were isolated as reducing oligosaccharides after hydrazinolysis/re-N-acetylation/mild acid treatment of the Pronase-digested glycoprotein. The sialylated oligosaccharides were separated by high-performance liquid chromatography in two different systems, which resulted in greater than 35 fractions, comprising di-, tri-, tetra-, and pentasialylated oligosaccharides. The major components were isomeric structures comprising the tri- and tetrasialylated fractions. In this and the accompanying paper (Cumming et al., 1989), the structures of 10 of the major components of the tri-, tetra-, a nd pentasialylated oligosaccharide fractions are described. Separation protocols and three isolated structures having sialic acid linked only to galactose are presented in this paper.