Characterization of a model of osteoarthritis in the rabbit knee
Characterization of a model of osteoarthritis in the rabbit knee
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DOI：
10.1016/s1063-4584(05)80318-8
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发表时间：
1996-06-01
影响因子：
7
通讯作者：
Hacker, SA
中科院分区：
文献类型：
--
作者：
Yoshioka, M;Coutts, RD;Hacker, SA
采用一种新的计算机化组织形态计量学方法评估兔骨关节炎（OA）模型的发展。三组10只新西兰白色家兔闭合骺，进行单侧前交叉韧带横断（ACLT）和对侧关节切开术（假手术）。各组分别于第4、8、12周处死。在死亡时，使用以下分级量表在涂抹印度墨水后对股骨髁进行大体评估。1级：表面完整; 2级：极轻微纤颤; 3级：明显纤颤; 4级：糜烂。使用彩色图像分析系统评估所有组织学切片。测量确定的软骨区域的平均厚度和面积。计算均方根表面粗糙度（基于与理想光滑表面的偏差），以评估关节软骨的表面轮廓。结果如下。ACLT后，4周时未观察到全层溃疡。4个内侧股骨髁在第8周和6个在第12周显示关节软骨全层溃疡。几乎所有区域的软骨面积百分比和软骨厚度（ACLT除以假手术）均随时间推移而降低，表明进行性侵蚀。ACLT组膝关节表面较假手术组粗糙。这些结果证明了使用计算机视频分析系统的定量方法在评估兔模型中ACLT后关节软骨OA发展的有用性。
A new computerized method of histomorphometry was used to assess the development of osteoarthritis (OA) in a rabbit model. Three groups of 10 New Zealand White rabbits with closed epiphyses underwent unilateral anterior cruciate ligament transection (ACLT) and contralateral arthrotomy (sham). Groups were killed at 4, 8 and 12 weeks. At the time of death the femoral condyles were assessed grossly following the application of India ink using the following grading scale. Grade 1: intact surface; grade 2: minimal fibrillation; grade 3: overt fibrillation; grade 4: erosion. All histological sections were assessed using a color image analysis system. The mean thickness and area were measured for a defined cartilage region. The root mean square surface roughness (based on deviations from an idealized smooth surface) was calculated to assess the surface profile of the articular cartilage. The results were as follows. After ACLT, no full-thickness ulceration was noted at 4 weeks. Four of the medial femoral condyles at 8 weeks and six at 12 weeks showed full-thickness ulceration of the articular cartilage. The per cent cartilage area and cartilage thickness (ACLT divided by sham) in almost all regions showed decreases with time, indicating progressive erosion. The surface of the ACLT knees was much rougher than that of sham of the knees. These results demonstrate the usefulness of a quantitative methodology using a computerized video analysis system to assess the articular cartilage following ACLT in a rabbit model for the development of OA.