Genetic Dissection of Seed Dormancy using Chromosome Segment Substitution Lines in Rice (Oryza sativa L.)
Genetic Dissection of Seed Dormancy using Chromosome Segment Substitution Lines in Rice (Oryza sativa L.)
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DOI：
10.3390/ijms21041344
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发表时间：
2020-02-01
影响因子：
5.6
通讯作者：
Yu, Sibin
中科院分区：
文献类型：
--
作者：
Yuan, Shaowen;Wang, Yuntong;Yu, Sibin
发芽的时间决定了一个新的植物生活史是否可以启动;因此，适当的休眠和在不同环境条件下的快速发芽是种子最重要的特征。然而，种子休眠和萌发行为的遗传结构仍然很难理解。以珍汕97为背景，利用146个携带日本晴（NIP）染色体片段的染色体片段代换系（CSSLs），对种子休眠和萌发行为进行了连锁分析。共检测到6个发芽参数的36个数量性状位点。其中，qDOM3.1近等基因系在分离群体中被验证为种子休眠性的主效QTL，并被进一步划定在第3染色体上90 kb的基因组区域内。基于遗传分析和基因表达谱，候选基因被限制到8个基因，其中4个响应于添加脱落酸（阿坝）。其中，LOC_Os03g01540参与阿坝信号通路，调控种子休眠。研究结果将有助于水稻等作物种子休眠和萌发的主效QTL的克隆和遗传结构的理解。
Timing of germination determines whether a new plant life cycle can be initiated; therefore, appropriate dormancy and rapid germination under diverse environmental conditions are the most important features for a seed. However, the genetic architecture of seed dormancy and germination behavior remains largely elusive. In the present study, a linkage analysis for seed dormancy and germination behavior was conducted using a set of 146 chromosome segment substitution lines (CSSLs), of which each carries a single or a few chromosomal segments of Nipponbare (NIP) in the background of Zhenshan 97 (ZS97). A total of 36 quantitative trait loci (QTLs) for six germination parameters were identified. Among them, qDOM3.1 was validated as a major QTL for seed dormancy in a segregation population derived from the qDOM3.1 near-isogenic line, and further delimited into a genomic region of 90 kb on chromosome 3. Based on genetic analysis and gene expression profiles, the candidate genes were restricted to eight genes, of which four were responsive to the addition of abscisic acid (ABA). Among them, LOC_Os03g01540 was involved in the ABA signaling pathway to regulate seed dormancy. The results will facilitate cloning the major QTLs and understanding the genetic architecture for seed dormancy and germination in rice and other crops.