Distribution of Lymphocyte Subpopulations in Thyroid Glands of Human Autoimmune Thyroid Disease
Distribution of Lymphocyte Subpopulations in Thyroid Glands of Human Autoimmune Thyroid Disease
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DOI：
10.1002/jcla.21674
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发表时间：
2014-05-01
影响因子：
2.7
通讯作者：
Wu, Guilong
中科院分区：
文献类型：
--
作者：
Zha, Bingbing;Huang, Xiuyan;Wu, Guilong
研究背景自身免疫性甲状腺疾病（AITD）是一种以自身免疫耐受性破坏为特征的器官特异性自身免疫性疾病。一些淋巴细胞已被确定与AITD的发病机制显著相关。本文评估了淋巴细胞亚群在甲状腺的分布，以开发免疫特异性形式的治疗AITD.MethodsDamaged甲状腺标本从18个格雷夫斯病（GD）和17个桥本甲状腺炎（HT）患者。正常甲状腺标本取自17例接受甲状旁腺切除术的患者的未受影响的腺体。通过免疫组化方法分析CD4+ T淋巴细胞、CD8+ T淋巴细胞、CD20+ B淋巴细胞及调节性T细胞（Treg）标志物FoxP3在甲状腺组织中的表达差异及相关性，评价甲状腺组织中淋巴细胞亚群的分布。18例GD甲状腺标本中有半数可见淋巴细胞浸润。其余9例GD标本中，CD8+ T细胞和CD20+ B细胞均有不同程度的表达，其中7例FoxP3+ T细胞阳性，2例CD4+ T细胞弱表达。17例HT患者甲状腺组织中CD20 + B细胞均呈强阳性，CD4+、CD8+ T细胞多或少表达，其中9例可见FoxP3+ T细胞。此外，基于FoxP3+ Tf3在一些AITD甲状腺标本中大量浸润，我们认为，与仅增加Tf3 '数量相比，激活Tf3'功能在某些情况下可能是治疗AITD的更有效方法。
BackgroundThe autoimmune thyroid disease (AITD) is an organ-specific autoimmune disease characterized by the breakdown of self-tolerance to thyroid antigens. Some lymphocytes have been identified to be related notably to the pathogenesis of AITD. This article evaluated the distribution of the lymphocytic subpopulation in thyroid glands in order to develop the immunospecific forms of therapy for AITD.MethodsDamaged thyroid specimens were obtained from 18 Graves' disease (GD) and 17 Hashimoto's thyroiditis (HT) patients. Normal thyroid specimens were obtained from unaffected glands of 17 patients who underwent parathyroidectomy. We evaluated the distribution of lymphocytic subpopulation by analyzing the expression difference and correlationship among CD4+ T lymphocyte, CD8+ T lymphocyte, CD20+ B lymphocyte as well as regulatory T cells(Tregs)' marker FoxP3 in the thyroid tissues via immunohistochemistry.ResultsOur research uncovered that no distinct lymphocyte infiltrated in the normal thyroid specimens. Scarcely any lymphocyte infiltration could be found in half of the totally 18 GD thyroid specimens. For the rest 9 GD specimens, CD8+ T cells and CD20+ B cells were expressed more or less in all of them, FoxP3+ Tregs were detected in 7 of them and CD4+ T cells were weakly expressed in only 2 of them. For the 17 HT thyroid specimens, CD20+ B cells were stained strongly in all of them, CD4+, CD8+ T cells were expressed more or less in most of them and FoxP3+ Tregs could be detected in 9 of them.ConclusionBased on CD20+ B cells predominantly infiltrating in all HT thyroid tissues we suggested CD20 antibody might be of help for HT treatment. Furthermore based on FoxP3+ Tregs abundantly infiltrating in some of the AITD thyroid specimens, we considered that activating the Tregs' function in comparison to increasing the Tregs' number only, may be a more effective approach to the treatment of AITD in some cases.