Cloning and expression of human apolipoprotein D cDNA.
Cloning and expression of human apolipoprotein D cDNA.
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DOI：
10.1016/s0021-9258(18)66599-8
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发表时间：
1986-12
期刊：
影响因子：
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通讯作者：
D. Drayna;C. Fielding;J. McLean;B. Baer;Graciela CastroS;E. Chen;L. Comstock;W. Henzel;W. Kohr;L. Rhee;K. Wion;R. Lawn
中科院分区：
文献类型：
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作者：
D. Drayna;C. Fielding;J. McLean;B. Baer;Graciela CastroS;E. Chen;L. Comstock;W. Henzel;W. Kohr;L. Rhee;K. Wion;R. Lawn
从克隆的cDNA序列中获得了人载脂蛋白D的氨基酸序列，载脂蛋白D是高密度脂蛋白的一个组分。该169个氨基酸的蛋白质与其他载脂蛋白序列没有明显的相似性，但与血浆视黄醇结合蛋白和α 2u-球蛋白超家族的其他成员具有高度的同源性。载脂蛋白D mRNA已在人肝、肠、胰腺、肾、胎盘、肾上腺、脾和胎儿脑组织中检测到。用克隆的cDNA转染的组织培养细胞分泌与抗apoD抗体反应的物质。
The amino acid sequence of human apolipoprotein D, a component of high density lipoprotein, has been obtained from the cloned cDNA sequence. The 169-amino acid protein has no marked similarity to other apolipoprotein sequences, but has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2u-globulin protein superfamily. Apolipoprotein D mRNA has been detected in human liver, intestine, pancreas, kidney, placenta, adrenal, spleen, and fetal brain tissue. Tissue culture cells transfected with the cloned cDNA secrete material that reacts with anti-apoD antibodies.